超级细菌NDM-1基因高通量微球悬浮芯片检测方法的建立

Establishment of detecting method of NDM-1 gene by microsphere suspension array

中文关键词:  超级细菌  NDM-1基因  微球悬浮芯片  检测  

英文关键词:super bactiria  metallo-beta-lactamase 1 gene  detection  microsphere suspension array  

中文摘要:

      本研究以建立微球体悬浮芯片检测方法为目的,选择三种超级细菌NDM-1基因(metallo-beta-lactamase 1 gene)为目的基因,设计并合成标记特异性探针,与荧光编码微球偶联后与细菌NDM-1基因的PCR产物杂交反应,用微球悬浮芯片检测仪检测荧光信号,建立可快速检测该种基因的微球体悬浮芯片检测方法。特异性和灵敏度检测结果表明:该方法对NDM-1基因检测具有特异性,最低检测限可达10fg/μL,灵敏度高于普通PCR技术,提示微球体悬浮芯片是一种针对NDM-1的快速、灵敏、便捷的新型核酸检测技术。

英文摘要:

      In this paper, a new method, which based on liquid chip to detect NDM-1 (metallo-beta-lactamase 1 gene) sample was established. A highly specific probe was synthesized and marked by biotin to target the NDM-1, coupled with fluorescent coded microspheres, and then hybridized with the PCR product of NDM-1 gene. Hybridization fluorescence signal was detected through microsphere suspension array (MSA). Specific and sensitivity were tested which were compared with PCR method. The result shows that the sensitivity of MSA was 10 fg/μl of vector DNA, which was higher than the result of PCR method. Detecting NDM-1 with MSA is specific and sensitive method.