| 吴兴海,张云霞,吴振兴,梁炜,鲍蕾,雷质文,梁成珠,徐彪.利用环介导等温扩增技术检测NDM-1基因的研究初探[J].食品安全质量检测学报,2011,2(3):125-129 |
| 利用环介导等温扩增技术检测NDM-1基因的研究初探 |
| The preliminary study of detection of NDM-1 by LAMP |
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| DOI： |
| 中文关键词: 环介导等温扩增技术 检测NDM-1基因 |
| 英文关键词:LAMP detection of NDM-1 |
| 基金项目:山东检验检疫局科技专项:食品中超级细菌耐药基因NDM-1基因多重复合痕量检测体系的建立(SK2008-19) |
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| Author | Institution |
| Wu Xinghai | Shandong Exit-Entry Inspection and Quarantine Bureau, Qingdao 266002, China |
| Zhang Yunxia | Shandong Exit-Entry Inspection and Quarantine Bureau, Qingdao 266002, China |
| Wu Zhenxing | Shandong Exit-Entry Inspection and Quarantine Bureau, Qingdao 266002, China |
| Liang Wei | Shandong Exit-Entry Inspection and Quarantine Bureau, Qingdao 266002, China |
| Bao Lei | Shandong Exit-Entry Inspection and Quarantine Bureau, Qingdao 266002, China |
| Lei Zhiwen | Shandong Exit-Entry Inspection and Quarantine Bureau, Qingdao 266002, China |
| Liang Chengzhu | Shandong Exit-Entry Inspection and Quarantine Bureau, Qingdao 266002, China |
| Xu Biao | Shandong Exit-Entry Inspection and Quarantine Bureau, Qingdao 266002, China |
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| 中文摘要: |
| 以人工合成的NDM-1基因序列为研究材料,在其保守区设计3对4条恒温扩增引物,通过特异性及灵敏度验证实验,初步建立了NDM-1基因的LAMP检测方法。琼脂糖凝胶电泳结果表明,研究中NDM-1基因经过等温扩增后形成了特异性的梯状条带,为食品中潜在超级细菌的现场快速检测提供了一种更加简便快速的方法,可以满足现场检疫的需要。 |
| 英文摘要: |
| The synthetic NDM-1 gene sequence as the research material,three pairs of primers was designed and a detection method of NDM-1 was developed preliminarily through specificity and sensitivity test. Agarose gel electrophoresis result showed special waterfall-like fragment was produced,and a rapid diagnosis of super bacteria was supplied for field inspection. |
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