食源性微生物快速检测研究进展

Research progress on fast detection methods of foodborne pathogenic microbe

中文关键词:  食源性微生物  快速检测  食品安全  

英文关键词:food pathogens  fast detection  food safety  

中文摘要:

      食源性微生物是影响食品安全的主要因素之一,本文回顾了食源性微生物引起的食源性疾病及对人类的危害;介绍了国内外7种食源性微生物快速检测技术的研究现状,指出常规的微生物专用酶快速反应检测技术、分析化学技术、载体技术、代谢学技术、免疫分析检测技术、分子生物检测技术以及其它新生物学技术检测方法,的灵敏度和专一性有一定提高,但操作繁琐;现在发展的分子生物学方法为食品中快速、灵敏的病毒检测带来了更多的希望,各种快速检测方法还只是实践中的一个参考。其今后的发展方向主要是:克服现有分子生物学方法的缺点,最好能定量测定微生物细胞特征活性能,简化程序,提高效率。

英文摘要:

      Pathogenic microbe was one of the most primary factor which effects on food safety.This article introduced food-borne illness and harm of world food safety,and State of the Field of fast detection about seven Pathogenic microbe,detection of reaction enzyme is low sensitivity.There had been heighten level on technically of Analytical chemistry、vector、metabonomics、immunoassay、molecular biology and others,but complicated operating instruction,there is a hole on methods in molecular biology,only reference.Orientation of technological development of microbiological detection:Cell-energy would be quantitative analysis, The subject is not immensely complex,and easy to simplify.