固相萃取-高效液相色谱法测定食品中痕量花粉红染料

Determination of Rhodamine B dyestuff in foods by solid-phase extraction-HPLC method

中文关键词:  高效液相色谱法  花粉红  中性氧化铝食品  测定  

英文关键词:HPLC  Rhodamine B  aluminium oxide neutral  food  determination  

中文摘要:

      本实验建立了测定食品中痕量花粉红色素的高效液相色谱方法。样品经正己烷等溶液提取,经过中性氧化铝柱净化,以甲醇和水溶液为流动相进行色谱分离,在激发波长550nm,发射波长580nm的荧光条件下检测。花粉红的浓度在0.5～10.0μg/L范围内与其色谱峰面积的线性关系良好(r=0.999),在1、5、10μg/kg添加水平时的回收率为56.3%～80.1%,RSD为2.8%～5.7%。方法的定量检出限为1.0μg/kg。

英文摘要:

      To develop a method for the quantitative analysis trace amount of Rhodamine B dyestuff in foods,the sample which was dissolved in n-hexane solution was extracted by adsorption with Alumina N-neutral column. HPLC was carried out on the Aluminium oxide neutral column with the fluorescence detector and methyl alcohol-water solution as mobile phase. The excitation wavelength is 550 nm,and the emission wavelength is 580nm. There was good linear relationship between the peak area and the sample content injected in the ranges of 0.5 μg/L～10.0 μg/L(r=0.999)for Rhodamine B. The recovery rate of Rhodamine B was between 56.3% and 80.1%,and RSD was 2.8%～5.7%. The detection limit is 1.0 μg/kg. The method is simple,accurate,and suitable for the determination of Rhodamine B dyestuff in foods.