| 高宏伟,徐彪,朱来华,肖西志,郑小龙,梁成珠.应用LAMP检测方法检测肉制品中的单增李斯特菌[J].食品安全质量检测学报,2010,1(1):12-17 |
| 应用LAMP检测方法检测肉制品中的单增李斯特菌 |
| Detection of Listeria monocytogenes in meat by loop-mediated isothermal amplification (LAMP) |
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| DOI： |
| 中文关键词: 单增李斯特菌 actA基因 环介导等温核酸扩增 |
| 英文关键词:Listeria monocytogenes actA gene LAMP |
| 基金项目:科技部科技支撑项目(2006BAD05A06-01) |
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| Author | Institution |
| Gao Hongwei | Technical Center for Shandong Entry-Exit Inspection and Quarantine Bureau,Qingdao 266002,China |
| Xu Biao | Technical Center for Shandong Entry-Exit Inspection and Quarantine Bureau,Qingdao 266002,China |
| Zhu Laihua | Technical Center for Shandong Entry-Exit Inspection and Quarantine Bureau,Qingdao 266002,China |
| Xiao Xizhi | Technical Center for Shandong Entry-Exit Inspection and Quarantine Bureau,Qingdao 266002,China |
| Zheng Xiaolong | Technical Center for Shandong Entry-Exit Inspection and Quarantine Bureau,Qingdao 266002,China |
| Liang Chengzhu | Technical Center for Shandong Entry-Exit Inspection and Quarantine Bureau,Qingdao 266002,China |
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| 中文摘要: |
| 本研究使用恒温环介导技术,以单增李斯特菌的肌动蛋白大会诱导蛋白聚集因子A(actA)基因设计引物,建立单增李斯特菌的LAMP快速检测方法。不同来源的13株单增李斯特菌LAMP检测均显示阳性,其他21种细菌LAMP检测显示阴性。使用使用本研究建立的LAMP方法检测肉类样品中的单增李斯特菌结果与细菌分离方法检测结果一致。 |
| 英文摘要: |
| A loop-mediated isothermal amplification(LAMP)method has been developed for the detection of L.monocytogenes isolates in both pure bacterial cultures and pork.The LAMP primers,which correspond to the actin-assembly inducing protein precursor gene(actA),successfully identify 13 different bioserotypes of L.monocytogenes but fail on 21 non-L.monocytogenes strains.The sensitivity of the LAMP detection in pure culture is 5.7 fg for genomic DNA. |
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