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| 黑木耳多糖的制备纯化及降血糖功能验证 |
| Preparation, Purification and Hypoglycemic Function Verification of Auricularia heimuer Polysaccharides |
| 投稿时间：2026-06-26 修订日期：2026-07-16 |
| DOI： |
| 中文关键词: 黑木耳 多糖 降血糖活性 制备 纯化 |
| 英文关键词:Auricularia heimuer Polysaccharide Hypoglycemic activity Preparation Purification |
| 基金项目:2024黑龙江省重大科技成果产业化项目(CG24037) |
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| 中文摘要: |
| 从黑木耳中制备高活性降血糖多糖并评价其活性。通过单因素与正交试验优化酶解工艺，获得最佳α-葡萄糖苷酶抑制活性。粗多糖经Sevage法脱蛋白后，依次通过DEAE-52纤维素柱和Sephacryl S-200 HR凝胶柱纯化，得到精制组分AAPS4s。最佳工艺下多糖得率为25.69%±0.43%，α-葡萄糖苷酶抑制率为76.38%±0.94%。AAPS4s多糖含量为95.63%±1.46%，对α-葡萄糖苷酶和α-淀粉酶的抑制率分别为72.56%±2.12%和41.90%±0.87，抑制类型均为混合型；对DPPH自由基、羟基自由基和超氧阴离子的清除率分别为57.39%±0.73%、64.99%±2.65%和38.41%±2.80%。本研究成功获得活性明确的黑木耳多糖组分AAPS4s，证实其具有显著的体外降血糖与抗氧化潜力，为黑木耳在降糖功能产品开发中的高值化应用提供了依据。 |
| 英文摘要: |
| ABSTRACT: Objective To prepare hypoglycemic polysaccharides with high activity from Auricularia heimuer-judae and evaluate their biological activity. Methods Single-factor experiments and orthogonal tests were adopted to optimize the enzymatic hydrolysis process for the maximum inhibitory activity against α-glucosidase. After deproteinization via the sevage method, the crude polysaccharides were sequentially purified using a DEAE-52 cellulose column and a Sephacryl S-200 HR gel filtration column to obtain the refined fraction AAPS4s. Results Under the optimal conditions, the polysaccharide yield reached 25.69%±0.43%, and the α-glucosidase inhibition rate was 76.38%±0.94%. The polysaccharide purity of AAPS4s was 95.63%±1.46%. The inhibition rates of AAPS4s against α-glucosidase and α-amylase were 72.56%±2.12% and 41.90%±0.87%, respectively, and both inhibitions belonged to mixed-type. The scavenging rates against DPPH radicals, hydroxyl radicals and superoxide anion radicals were 57.39%±0.73%, 64.99%±2.65% and 38.41%±2.80%, respectively. Conclusion The purified polysaccharide fraction AAPS4s with definite activity was successfully isolated in this study. It exhibited significant in vitro hypoglycemic and antioxidant capacities. This research provides a theoretical foundation for high-value utilization of Auricularia heimuer in the development of hypoglycemic functional foods. |
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