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| 复杂基质保健酒中低含量总蒽醌的检测方法优化及验证 |
| Optimization and validation of detection method for low-content total anthraquinones in complex matrix health wine |
| 投稿时间：2026-05-19 修订日期：2026-07-31 |
| DOI： |
| 中文关键词: 总蒽醌 保健酒 分光光度法 方法优化 何首乌 |
| 英文关键词:total anthraquinones health wine spectrophotometry method optimization Polygoni Multiflori Radix |
| 基金项目:湖北省重点研发计划项目(2024BBB030) |
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| Author | Institution |
| SHI Hao | Jing Brand Co,Ltd |
| MAO Qiong-Li | Jing Brand Co,Ltd |
| LI Xian-Zhi | Jing Brand Co,Ltd |
| XIONG Xiao-Tong | Jing Brand Co,Ltd |
| YANG Feng | Jing Brand Co,Ltd |
| ZHU Yan | Jing Brand Co,Ltd |
| YAN Ling | Jing Brand Co,Ltd |
| LIU Yang | Jing Brand Co,Ltd |
| LI Qiang | Jing Brand Co,Ltd |
| YANG Qiang | Jing Brand Co,Ltd |
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| 中文摘要: |
| 目的 建立一种适用于复杂基质保健酒中低含量总蒽醌的准确、灵敏、抗干扰的检测方法。方法 以《保健食品理化及卫生指标检验与评价技术指导原则(2020年版)》分光光度法为基础，通过优化取样量、全量萃取、缩小定容体积、离心净化等前处理步骤，降低基质干扰并提高灵敏度; 以2025版《中国药典》何首乌项下高效液相色谱法为参比方法进行验证，采用Bland-Altman分析评价结果的一致性。结果 优化后的方法在0~0.016 mg/mL范围内线性关系良好，线性决定系数r2为0.99998，检出限为0.009 mg/100 g，定量限为0.026 mg/100 g; 3个浓度水平的平均加标回收率为98.94%，相对标准偏差为1.58%。选取10批样品分别使用优化后的分光光度法与高效液相色谱法参比方法进行测定，Bland-Altman一致性分析结果表明，两种方法检测结果趋势一致。结论 该方法操作简便、灵敏度高、抗基质干扰能力强、结果准确可靠，适用于复杂基质保健酒中低含量总蒽醌的快速测定与质量控制。 |
| 英文摘要: |
| Objective To establish an accurate, sensitive and anti-interferential method for the determination of low-content total anthraquinones in health wine with complex matrix. Methods Based on the spectrophotometry method specified in 2020 Edition of the Technical Guiding Principles for Inspection and Evaluation of Physicochemical and Hygienic Indicators of Health Food, the sample pretreatment was optimized by adjusting sample weight, performing full-volume extraction, reducing the constant volume, and adopting centrifugal purification to minimize matrix interference and enhance detection sensitivity. The optimized method was verified using the high performance liquid chromatography method under the item of Polygoni Multiflori Radix in the 2025 edition of the Chinese Pharmacopoeia as the reference method, and the consistency of the results was evaluated by Bland-Altman analysis. Results The optimized method exhibited good linearity in the range of 0~0.016 mg/mL with a correlation coefficient of 0.99998. The limit of detection was 0.009 mg/100 g, and the limit of quantitation was 0.026 mg/100 g. The average spiked recovery at three concentration levels was 98.94%, with a relative standard deviation of 1.58%. The 10 batches of samples were determined by the optimized spectrophotometry and the high performance liquid chromatography reference method. The Bland-Altman consistency analysis indicated that the detection results of the two methods exhibited consistent trends. Conclusion The method is simple, sensitive, robust against matrix interference, and accurate. It is suitable for the rapid determination and quality control of low-content total anthraquinones in health wine with complex matrix. |
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