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| 乳酸片球菌TC-4的培养基优化及冻干保护剂筛选 |
| Optimization of the culture medium for Pediococcus acidilactici TC-4 and screening of freeze-drying protective agents |
| 投稿时间：2026-03-19 修订日期：2026-05-30 |
| DOI： |
| 中文关键词: 乳酸片球菌 高尿酸血症 培养基 冻干保护剂 |
| 英文关键词:Pediococcus acidilactici hyperuricemia culture medium freeze-drying protective agent |
| 基金项目:国家重点研发专项(2023YFF1104100); 广东省重点研发专项(2022B111070006)；广东省科学院项目(2022GDASZH-2022010101) |
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| Author | Institution |
| ZHANG Zi-Yao | College of Food Science,Northeast Agricultural University |
| HAO Yu | College of Food Science,Northeast Agricultural University |
| CHEN Ling | Guangdong Institute of Microbiology, Guangdong Academy of Sciences, State Key Laboratory of Applied Microbiology, Guangdong Provincial Key Laboratory of Microbial Safety and Health, National Health Commission Key Laboratory of Microbial Food Nutrition and Safety, National Market Supervision and Administration Key Laboratory (Big Data Technology for Food Microbial Safety;Guangdong Microbial Analysis and Detection Center |
| LI Ying | Guangdong Institute of Microbiology, Guangdong Academy of Sciences, State Key Laboratory of Applied Microbiology, Guangdong Provincial Key Laboratory of Microbial Safety and Health, National Health Commission Key Laboratory of Microbial Food Nutrition and Safety, National Market Supervision and Administration Key Laboratory (Big Data Technology for Food Microbial Safety;Guangdong Microbial Analysis and Detection Center |
| XIE Xin-Qiang | Guangdong Institute of Microbiology, Guangdong Academy of Sciences, State Key Laboratory of Applied Microbiology, Guangdong Provincial Key Laboratory of Microbial Safety and Health, National Health Commission Key Laboratory of Microbial Food Nutrition and Safety, National Market Supervision and Administration Key Laboratory (Big Data Technology for Food Microbial Safety) |
| LIANG Ting-Ting | Guangdong Institute of Microbiology, Guangdong Academy of Sciences, State Key Laboratory of Applied Microbiology, Guangdong Provincial Key Laboratory of Microbial Safety and Health, National Health Commission Key Laboratory of Microbial Food Nutrition and Safety, National Market Supervision and Administration Key Laboratory (Big Data Technology for Food Microbial Safety |
| MAN Chao-Xin | College of Food Science,Northeast Agricultural University |
| WU Qing-Ping | Guangdong Institute of Microbiology, Guangdong Academy of Sciences, State Key Laboratory of Applied Microbiology, Guangdong Provincial Key Laboratory of Microbial Safety and Health, National Health Commission Key Laboratory of Microbial Food Nutrition and Safety, National Market Supervision and Administration Key Laboratory (Big Data Technology for Food Microbial Safety) |
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| 中文摘要: |
| 目的 推动具有缓解高尿酸血症(Hyperuricemia, HUA)作用的乳酸片球菌 TC-4 从研究走向应用、实现产业化发展，开展其培养基组分及冻干保护剂配方的优化研究。方法? 采用单因素试验、Plackett-Burman 试验与响应面法相结合，对乳酸片球菌 TC-4培养基的成分、浓度以及冻干保护剂的配方展开筛选。结果 最优培养基成分组合:葡萄糖26 g/L、酵母浸粉30 g/L，乙酸钠8 g/L、柠檬酸氢二铵2 g/L、碳酸氢钾2 g/L、硫酸镁0.4 g/L、硫酸锰0.075 g/L、吐温80 1 g/L，活菌数由3.5×109 CFU/mL提高至1.09×1010 CFU/mL，是优化前的3.11倍。最优冻干保护剂配方:L-谷氨酸钠8%，麦芽糖醇14%，脱脂乳粉18%，存活率达到90.77%。结论? 完成乳酸片球菌TC-4的培养基优化，成功制备高活菌制剂，为该菌株的产业化推进奠定坚实理论基础。 |
| 英文摘要: |
| ABSTRACT:Objective To promote the application of Pediococcus acidilactici TC-4, which has the effect of alleviating hyperuricemia (HUA), from research to industrialization, and to carry out the optimization research on the components of its culture medium and the formula of freeze-drying protectants. Methods? The composition and concentration of the culture medium for Pediococcus acidilactici TC-4 and the formula of the freeze-drying protectant were screened by combiningsingle combiningsingle-factor experiments, Plackett-Burman experiments and response surface methodology |
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