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| 微波消解-高效液相色谱-蒸发光散射法测定谷物中的总阿拉伯木聚糖含量 |
| Determination of total arabinoxylan content in cereals by microwave digestion - high performance liquid chromatography - evaporation light scattering detection |
| 投稿时间：2026-03-10 修订日期：2026-05-14 |
| DOI： |
| 中文关键词: 总阿拉伯木聚糖 谷物 微波消解 高效液相色谱 蒸发光散射 |
| 英文关键词:total arabinoxylan cereals microwave digestion high performance liquid chromatography evaporative light scattering detection |
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| 摘要点击次数: 22 |
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| 中文摘要: |
| 目的 建立一种测定谷物中总阿拉伯木聚糖含量的微波消解-高效液相色谱(high performance liquid chromatography, HPLC)-蒸发光散射(evaporative light scattering detection, ELSD)分析方法。方法 试样经1.0 mol/L三氟乙酸溶液120 ℃微波消解30 min, 除酸复溶后分析。采用NH2色谱柱（4.6 mm×250 mm, 5 μm）分离, 以乙腈-水（90:10, V:V）洗脱, 蒸发光散射检测器检测, 外标法定量, 并对常见谷物样品进行检测分析。结果 经方法学验证, 木糖、阿拉伯糖在0.2~2.0 mg/mL范围内线性关系良好, 决定系数均大于0.999, 检出限分别为1.0 mg/g、1.5 mg/g, 定量限分别为2.5 mg/g、5.0 mg/g, 回收率为86.8%~93.1%, 相对标准偏差均小于3.5%。结论 该方法准确、可靠、灵敏, 适用于谷物中总阿拉伯木聚糖的测定，可为谷物品质评价、加工适应性分析及膳食营养研究提供参考。 |
| 英文摘要: |
| Objective To establish a method for the determination of total arabinoxylan in cereals, microwave digestion combined with high performance liquid chromatography (HPLC)-evaporative light scattering detection (ELSD). Methods Following digestion with 1.0 mol/L trifluoroacetic acid at 120 ℃ for 30 min, the samples were de-acidified, re-dissolved and subsequently analyzed. Separation was performed on an NH2 chromatographic column (4.6 mm×250 mm, 5 μm) using acetonitrile-water (90:10, V:V) as the mobile phase. Detection was conducted using an evaporative light scattering detector, quantification was performed by the external standard method, and the developed method was applied to the analysis of common cereal samples. Results Good linearity was observed for xylose and arabinose in the range of 0.2 to 2.0 mg/mL, with coefficient of determination exceeding 0.999. The limits of detection were 1.0 mg/g for xylose and 1.5 mg/g for arabinose, and the corresponding limits of quantitation were 2.5 mg/g and 5.0 mg/g. Recoveries ranged from 86.8% to 93.1% (relative standard deviation<3.5%). Conclusion The method is accurate, reliable, and sensitive, making it suitable for the determination of total arabinoxylan in cereals and providing a reference for cereal quality evaluation, processing adaptability analysis, and dietary nutrition research. |
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