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| 泡叶藻专用复合酶制剂研发及应用 |
| Research and Application of Compound Enzyme Preparation of Ascophyllum nodosum |
| 投稿时间：2026-03-02 修订日期：2026-05-13 |
| DOI： |
| 中文关键词: 泡叶藻 海藻酸钠 褐藻胶裂解酶 复合酶制剂 褐藻寡糖 |
| 英文关键词:Ascophyllum nodosum Sodium alginate Alginate lyase Composite enzyme preparation Alginate oligosaccharide |
| 基金项目:山东省重点研发计划(2023CXGC010414、2022SFGC0101) |
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| 摘要点击次数: 36 |
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| 中文摘要: |
| 目的 研究褐藻胶裂解酶E1的最佳应用条件,建立针对泡叶藻的专用复合酶制剂最佳工艺条件。方法 通过控制变量法确定褐藻胶裂解酶E1的最适条件,从褐藻胶裂解酶E1的最适条件出发,以泡叶藻为研究对象,以纤维素酶、果胶酶、木瓜蛋白酶作为复配酶,通过泡叶藻酶解响应面试验,获得泡叶藻复合酶制剂的最佳工艺条件,以该条件对不同地理群体的泡叶藻(秘鲁、智利、法国)进行酶解,以褐藻寡糖提取率为指标进行分析验证该复合酶制剂对不同来源的泡叶藻的降解作用,比较酶解产物褐藻寡糖提取率及固体转化率,验证该复合酶制剂的酶解效果。结果 褐藻胶裂解酶E1的最佳应用条件为加酶量0.5%,pH=7.5,作用时间18 h,酶解温度45 ℃,泡叶藻专用复合酶制剂最佳工艺条件为纤维素酶1.82%,果胶酶1.2%,木瓜蛋白酶1.5%,褐藻胶裂解酶0.6%,料液比1:25,酶解温度45 ℃,pH=7.22,作用时间18 h,在此条件下,褐藻寡糖提取率为14.46%,固体转化率为65.3%,样品前处理优化后固体转化率可提升至68.6%。结论 通过本文复合酶制剂对不同来源的泡叶藻的降解作用分析,发现该复合酶制剂对不同来源的泡叶藻均具有有效降解作用且该复合酶制剂与本次实验优化后商业化复合酶制剂具有相当的酶解效果,并明显高于优化前商业化复合酶制剂与单一褐藻胶裂解酶的酶解效果。 |
| 英文摘要: |
| Objective Study the optimal application conditions for alginate lyase E1 and establish the best process conditions for a specialized composite enzyme preparation targeting Ascophyllum Nodosum. Methods The optimal conditions for alginate lyase E1 were determined using the control variable method. Starting from these optimal conditions, Ascophyllum Nodosum was selected as the research subject, with cellulase, pectinase, and papain used as composite enzymes. Through response surface methodology experiments on enzymatic hydrolysis of Ascophyllum Nodosum, the best process conditions for the composite enzyme preparation were obtained. Under these conditions, the degradation effect of the composite enzyme preparation on Ascophyllum Nodosum from different geographical populations (Peru, Chile, France) using this composite enzyme preparation was analyzed, the extraction rates of alginate oligosaccharide as indicators. By comparing the extraction rate of alginate oligosaccharides and solid conversion rate of enzymatic hydrolysis products to validate the hydrolysis efficiency of the composite enzyme preparation. Results The optimal application conditions for the alginate lyase E1, with an enzyme dosage of 0.5%, pH 7.5, reaction time 18 hours, and hydrolysis temperature 45 ℃. The optimal process conditions for the composite enzyme preparation of Ascophyllum Nodosum enzymatic hydrolysis are cellulase 1.82%, pectinase 1.2%, papain 1.5%, and alginate lyase 0.6%, solid-liquid ratio was 1:25, with an enzymatic hydrolysis temperature of 45 ℃, pH 7.22, and a reaction time of 18 hours. Under these conditions, the extraction rates of alginate oligosaccharide is 14.46%, the solid conversion rate is 65.3%, and through sample pretreatment optimization, the solid conversion rate can be increased to 68.6%. Conclusion Through the analysis of the degradation effect of the composite enzyme preparation on different sources Ascophyllum Nodosum of in this article, it was found that the composite enzyme preparation has effective degradation effects on different sources of Ascophyllum Nodosum and the composite enzyme preparation had a comparable enzymatic hydrolysis effect to the optimized commercial composite enzyme preparation in this experiment, and was significantly higher than the enzymatic hydrolysis effect of the commercial composite enzyme preparation before optimization and the single alginate lyase. |
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