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| 基于高效液相色谱-串联四极杆静电场轨道阱高分辨质谱 的代谢组学技术分析不同产地枸杞的差异代谢物 |
| Analysis of differential metabolites in geographical indication products of Lycium Barbarum L. from different origins by HPLC-Q-Orbitrap HRMS metabolomics technology |
| 投稿时间：2026-03-01 修订日期：2026-08-16 |
| DOI： |
| 中文关键词: 甘肃枸杞 非靶向代谢组学 差异代谢物 代谢通路 |
| 英文关键词:Lycium barbarum L. from Gansu non-targeted metabolomics characteristic differential metabolites metabolic pathways |
| 基金项目:甘肃省市场监督管理局科技计划项目（SSCJG-SP-A202302） |
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| 中文摘要: |
| 目的 以不同产地的甘肃省地理标志保护产品枸杞为研究对象,采用非靶向代谢组学技术探究不同产地枸杞的差异性标志代谢物。方法 通过采集甘肃靖远县和甘肃民勤县的枸杞样本,利用高效液相色谱-串联四级杆轨道阱质谱(HPLC-Q-Exactive Orbitrap /MS)进行非靶向代谢组学分析。采用主成分分析和正交偏最小二乘判别分析等方法,解析两种不同产地枸杞的差异代谢物。结果 靖远枸杞和民勤枸杞共有102种差异代谢物,上调39种,下调63种。差异代谢物以黄酮类为主,其次为酚酸类及其衍生物、氨基酸及其衍生物等。麦角新碱、组氨醇、脱氢-β-紫罗兰酮等为特征差异物。KEGG结果显示,两产地枸杞的差异代谢物主要富集在黄酮类化合物生物合成,黄酮和黄酮醇的生物合成,苯丙烷类物质生物合成,叶酸一碳池代谢,丙氨酸、天冬氨酸和谷氨酸代谢等代谢通路上。结论 研究结果为不同产地枸杞的品质差异和产地鉴别提供参考依据,同时也为枸杞的质量评价提供了一种新思路。 |
| 英文摘要: |
| ABSTRACT: Objective To explore the differential marker metabolites of geographical indication protected products of Lycium barbarum L. from different origins in Gansu Province by non-targeted metabolomics technology. Methods Non-targeted metabolomics analysis was conducted on Lycium barbarum L. samples collected from Jingyuan County and Minqin County in Gansun Province using high-performance liquid chromatography-tandem quadrupole orbitrap mass spectrometry (HPLC-Q-Exactive Orbitrap/MS). Principal component analysis and orthogonal partial least squares-discriminant analysis were employed to identify differential metabolites between the two origins. Results There are a total of 102 significantly differential metabolites between Jingyuan and Minqin Lycium barbarum L., among which 39 were up-regulated and 63 were down-regulated. Flavonoids constituted the predominant category of differential metabolites, followed by phenolic acids and their derivatives, amino acids and their derivatives, among others. Characteristic differential compounds included ergometrine, histaminol, and dehydro-β-ionone. KEGG pathway analysis indicated that the differential metabolites were primarily enriched in metabolic pathways such as flavonoid biosynthesis, flavone and flavonol biosynthesis, phenylpropanoid biosynthesis, one-carbon pool by folate, and alanine, aspartate, and glutamate metabolism. Conclusions These findings provide a reference for understanding quality variations and geographical origin identification of Lycium barbarum L. from different regions, while also offering a novel approach for quality assessment of Lycium barbarum L.. |
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