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| OIBW64特殊样品水平衡仪与稳定同位素比值质谱联用技术测定蜂蜜水中氧稳定同位素比值应用研究 |
| Application research on the determination of oxygen stable isotope ratio in water of honey by OIBW64-Isotope Ratio Mass Spectrometer |
| 投稿时间：2026-02-11 修订日期：2026-05-28 |
| DOI： |
| 中文关键词: 蜂蜜 水中δ18O值 OIBW64特殊样品水平衡仪与稳定同位素比值质谱联用技术 产地溯源 掺假鉴别 |
| 英文关键词:honey δ18O value in water OIBW64-isotope ratio mass spectrometer origin traceability adulteration identification |
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| 中文摘要: |
| 目的 建立一种高效、准确测定蜂蜜水中δ18O值的方法, 并系统评估该方法在蜂蜜地理溯源与掺假鉴别中的适用性。方法 基于OIBW64特殊样品水平衡仪与稳定同位素比值质谱联用技术(OIBW64-Isotope Ratio Mass Spectrometer, OIBW64-IRMS), 建立蜂蜜水中δ18O值的测定方法, 通过乙醇溶解、CO2-H2O平衡及自动进样系统, 实现样品前处理与测定流程的一体化与自动化。通过模拟掺假实验研究蜂蜜水中δ18O值与掺假量的相关性, 并分析了我国11个省份、涵盖9个品种的31份蜂蜜样品。结果 方法学验证表明, 该方法进样稳定性好、反应体系气密性高、乙醇及样品含水量对测定结果无影响, 且测定结果与主流分析技术高度一致[决定系数(r-squared, r2)>0.999]。模拟掺假实验中, 蜂蜜水中δ18O值与大米糖浆添加比例呈高度线性相关（Y=0.0956X-8.0719, r2=0.9903）, 该技术具有鉴定掺假与定量分析的可能性。样品分析显示, 不同产地蜂蜜的水中δ18O值存在显著地理差异, 且蜜源植物蒸腾特征、采蜜期、成熟度等也会对其水中δ18O产生影响。结论 本研究建立的OIBW64-IRMS方法稳定可靠, 准确性高, 适用于蜂蜜的水中δ18O值分析。蜂蜜水中δ18O值不仅显示出其在鉴别C3植物源糖浆掺假方面的应用潜力, 也可作为表征产地特征、植物特性的潜在指标, 为研究蜂蜜产品的真实性问题提供了新的技术手段。 |
| 英文摘要: |
| Objective Establish an efficient and accurate method for determining δ18O values in water of honey, and systematically evaluate the applicability of this method in the geographical traceability and adulteration identification of honey. Methods A method for determining δ18O values in water of honey, was established using OIBW64-Isotope Ratio Mass Spectrometer (OIBW64-IRMS). Sample preparation and analysis were integrated and automated through ethanol dissolution, headspace CO2-H2O equilibrium, and an automated sample introduction system. The correlation between the δ18O value in water of honey and the amount of adulteration was studied through a simulated adulteration experiment. This method was also applied to analyze 31 honey samples from 11 provinces across China, covering 9 varieties. Results Methodological validation shows that this method has good injection stability, high gas tightness of the reaction system, no influence of ethanol and sample moisture content on the determination results, and the determination results are highly consistent with the mainstream analytical techniques [coefficient of determination (r-squared, r2)>0.999]. In the simulated adulteration experiment, there was a highly linear correlation between the δ18O value in water of honey and the proportion of rice syrup added (Y=0.0956X-8.0719, r2=0.9903). This technique has the potential to identify adulteration and perform quantitative analysis. Sample analysis shows that there are significant geographical differences in the δ18O values in water of honey from different origins, and the transpiration characteristics, honey harvesting period, maturity, and other factors of honey source plants can also affect their δ18O values in water. Conclusion The OIBW64-IRMS method established in this study is stable, reliable and highly accurate, and is suitable for the analysis of δ18O values in water of honey. The δ18O value in water of honey not only demonstrates its potential application in identifying adulteration of C3 plant-based syrup, but also serves as a potential indicator for characterizing the characteristics of the production area and plants. This provides a new technical means for studying the authenticity of honey products. |
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