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| 红法夫酵母中游离虾青素检测与提取工艺研究 |
| Detection of Free Astaxanthin and Optimization of the Extraction Process from Xanthophyllomyces dendrorhous |
| 投稿时间：2026-01-30 修订日期：2026-05-14 |
| DOI： |
| 中文关键词: 红法夫酵母 游离虾青素 二元梯度高效液相色谱法 提取工艺 |
| 英文关键词:Xanthophyllomyces dendrorhous (X. dendrorhous) free astaxanthin binary gradient high performance liquid chromatography extraction process |
| 基金项目:国家重点研发计划课题(2024YFD241604)；山东省现代农业虾蟹产业技术体系(SDARS-13-01)。3)第一作者信息崔函祎(2001-),女,硕士研究生,主要研究方向食品微生物,E-mail13345066328@163.com。4)通信作者信息牟海津(1973-),男,博士,教授,主要研究方向海洋微生物工程,E-mailmousun@ouc.edu.cn。刘天红(1982-),女,硕士,副研究员,主要研究方向水产品安全与质量控制,E-mailoucthl@126.com |
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| 中文摘要: |
| 目的 对游离虾青素液相检测方法进行优化，并开发红法夫酵母粉中虾青素的提取工艺。方法 通过优化检测时间与流动相比例，建立了基于高效液相色谱法（high performance liquid chromatography, HPLC）的游离虾青素分析方法。在此基础上，以红法夫酵母粉为原料，采用单因素实验、Plackett-Burman实验及响应面法，以虾青素提取率为指标，开发红法夫酵母粉中虾青素的提取工艺。结果 该方法检出限为0.55 μg/g （S/N=3），定量限为0.16 μg/g （S/N=10）；加标回收率为105.12% ~ 127.70%；精密度试验的相对标准偏差小于0.14%，表明该方法灵敏度高、准确性好、重复性强，满足游离虾青素的定量分析要求。开发并优化了红法夫酵母粉中虾青素的提取工艺，红法夫酵母超微粉平均粒径0.55 μm，水添加比例为3.30:1（mL/g），二甲基亚砜（dimethyl sulfoxide，DMSO）添加比例为41.25:1（mL/g），丙酮的添加比例为137.50:1（mL/g），提取次数2次，加水后52℃保温5 min，加DMSO后50℃保温5.5 min，提取率为2.74 mg/g。结论 本研究建立的游离虾青素HPLC检测方法灵敏、准确、重复性好，可实现红法夫酵母中游离虾青素的快速定量分析；优化的红法夫酵母粉中游离虾青素提取工艺具有高效、稳定、绿色的优势，为红法夫酵母虾青素在水产、食品等领域的应用提供技术支撑。 |
| 英文摘要: |
| Objective To optimize the liquid chromatography method for free astaxanthin detection and to develop an extraction process for astaxanthin from Xanthophyllomyces dendrorhous powder. Methods By optimizing the detection time and mobile phase ratio, an analytical method for free astaxanthin based on high performance liquid chromatography (HPLC) was established. On this basis, using X. dendrorhous powder as raw material, the extraction process for astaxanthin was developed via single?factor experiments, Plackett?Burman design, and response surface methodology, using astaxanthin extraction yield as the evaluation index. Results The limit of detection (LOD) of this method was 0.55 μg/g (S/N=3), and the limit of quantitation (LOQ) was 0.16 μg/g (S/N=10). The spike recoveries ranged from 105.12% to 127.70%, and the relative standard deviation (RSD) in the precision test was less than 0.14%, indicating that the method exhibits high sensitivity, good accuracy, and strong repeatability, meeting the requirements for quantitative analysis of free astaxanthin. The extraction process for astaxanthin from X. dendrorhous powder was developed and optimized as follows: the average particle size of superfine X. dendrorhous powder was 0.55?μm; the water addition ratio was 3.30:1 (mL/g); the dimethyl sulfoxide (DMSO) addition ratio was 41.25:1 (mL/g); the acetone addition ratio was 137.50:1 (mL/g); the extraction was performed twice; after water addition, the mixture was incubated at 52?℃ for 5?min; and after DMSO addition, it was incubated at 50?℃ for 5.5?min. The extraction yield was 2.74?mg/g. Conclusion The established HPLC method for free astaxanthin was sensitive, accurate, and reproducible, enabling rapid quantitative analysis of free astaxanthin in X. dendrorhous. The optimized extraction process was efficient, stable, and environmentally friendly. This study provided technical support for the application of X. dendrorhous astaxanthin in aquaculture, food, and other fields. |
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