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| 裂殖壶菌产二十二碳六烯酸-二十碳五烯酸油脂的遗传毒性及降脂活性评价 |
| Evaluation of genotoxicity and hypolipidemic activity ofdocosahexaenoic acid - eicosapentaenoic acid-rich oil from Schizochytrium |
| 投稿时间：2026-01-29 修订日期：2026-08-06 |
| DOI： |
| 中文关键词: 裂殖壶菌 二十二碳六烯酸 二十碳五烯酸 遗传毒性 降脂活性 3T3-L1前脂肪细胞 |
| 英文关键词:Schizochytrium sp. docosahexaenoic acid eicosapentaenoic acid genotoxicity hypolipidemic activity 3T3-L1 preadipocytes |
| 基金项目:福建省科技计划农业引导性（重点）项目，2024N0022；福建省科技计划引导性项目，2024Y0077 |
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| Author | Institution |
| Xia Yuyu | College of Food Science, Fujian Agriculture and Forestry University; Technology Innovation Center for Exploitation of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources |
| Wang Yun | Xiamen Food and Drug Quality Inspection Research Institute |
| Feng Yutong | Technology Innovation Center for Exploitation of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources |
| Chen Sisi | Technology Innovation Center for Exploitation of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources |
| Huang Shixin | Technology Innovation Center for Exploitation of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources |
| Chen Xiaolu | Technology Innovation Center for Exploitation of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources |
| Lin Hetong | College of Food Science, Fujian Agriculture and Forestry University |
| He Jianlin | Technology Innovation Center for Exploitation of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources |
| Hong Bihong | Technology Innovation Center for Exploitation of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources |
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| 中文摘要: |
| 目的 对裂殖壶菌产二十二碳六烯酸-二十碳五烯酸油脂(docosahexaenoic acid - eicosapentaenoic acid -rich oil from Schizochytrium, DHA-EPA SO) (DHA含量: 37.33±0.15%; EPA含量: 5.82±0.03%)进行遗传毒性与体外降脂活性评价，明确其安全性与功能性。方法 采用细菌回复突变试验、哺乳动物红细胞微核试验、小鼠精母细胞染色体畸变试验系统评价DHA-EPA SO的遗传毒性; 以3T3-L1前脂肪细胞为模型，通过油红O染色定性观察、吸光度定量分析及甘油三酯（triacylglycerol, TG）、总胆固醇（total cholesterol, TC）含量检测，探究DHA-EPA SO的体外降脂活性。结果 DHA-EPA SO在试验剂量范围内，三项遗传毒性试验结果均为阴性，其中细菌回复突变试验各菌株的回变菌落数均未超过阴性对照组2倍，小鼠骨髓红细胞微核率、精母细胞染色体畸变率均与对照组无显著差异(P>0.05)，无致突变性及遗传损伤风险；体外降脂试验显示，DHA-EPA SO可有效抑制3T3-L1细胞脂质沉积，降低细胞内TG、TC含量，浓度为12.5 μg/mL时呈现出最佳降脂效应，提高剂量时降脂活性有所减弱。结论 DHA-EPA SO无遗传毒性，同时具备显著的体外降脂活性，在功能性食品、保健品领域具有广阔应用前景。 |
| 英文摘要: |
| Objective To evaluate the genetic toxicity and in vitro hypolipidemic activity of docosahexaenoic acid - eicosapentaenoic acid -rich oil from Schizochytrium (DHA-EPA SO) (DHA content: 37.33±0.15%; EPA content: 5.82±0.03%), clarify its safety and functional properties. Methods Using the bacterial reverse mutation assay, mammalian erythrocyte micronucleus test and mouse spermatocyte chromosome aberration test, the genetic toxicity of DHA-EPA SO was systematically evaluated; Using 3T3-L1 preadipocytes as the cell model, the in vitro hypolipidemic activity of DHA-EPA SO was investigated via qualitative observation by Oil Red O staining, quantitative analysis of optical density value, and determination of intracellular triacylglycerol (TG) and total cholesterol (TC) contents. Results Within the test dose range, DHA-EPA SO presented negative results in three genetic toxicity assays. In the bacterial reverse mutation assay, the number of revertant colonies of each strain did not exceed twice that of the negative control group; the micronucleus rate of mouse bone marrow erythrocytes and the chromosome aberration rate of mouse spermatocytes showed no significant differences compared with the control groups(P>0.05), indicating no mutagenicity or genetic damage risk of DHA-EPA SO. The in vitro hypolipidemic tests demonstrated that DHA-EPA SO could effectively inhibit lipid accumulation in 3T3-L1 cells and reduce intracellular TG and TC contents, exerting the optimal hypolipidemic effect at a concentration of 12.5 μg/mL, while the hypolipidemic activity was attenuated with increased doses. Conclusion DHA-EPA SO has no genetic toxicity and exhibits significant in vitro hypolipidemic activity, which endows it with broad application prospects in the field of functional foods and health products. |
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