超声辅助纤维素酶法提取蜈蚣草多糖的工艺优化及降血糖血脂作用研究

Study on the optimization of ultrasound-assisted cellulase extraction for Pteris vittata polysaccharides and its hypoglycemic and hypolipidemic effects

中文关键词:  蜈蚣草  多糖  超声辅助纤维素酶法  降血糖  降血脂

英文关键词:Pteris vittata  polysaccharides  ultrasound-assisted cellulase extraction  hypoglycemic  hypolipidemic

中文摘要:

      目的 研究超声辅助纤维素酶法对蜈蚣草多糖的提取效果, 并探究其降血糖血脂活性。方法 采用响应面优化蜈蚣草多糖超声辅助纤维素酶法提取工艺参数, 并通过研究其对α-淀粉酶和α-葡萄糖苷酶活性抑制率评估体外降血糖活性, 通过研究其对胰脂肪酶抑制活性抑制率与甘氨胆酸盐和牛磺胆酸盐结合率评估体外降血脂活性。结果 蜈蚣草多糖最优提取工艺参数: 料液比1:20 (g:mL)、纤维素酶用量2.2%、超声功率 316 W、超声温度51 ℃、超声时间22 min, 在此条件下测得蜈蚣草多糖提取率为4.19%±0.08%。体外活性评价表明, 蜈蚣草多糖具有一定的降血糖和降血脂生物学活性, 蜈蚣草多糖对α-淀粉酶和α-葡萄糖苷酶的半数抑制浓度(half maximal inhibitory concentration, IC50)分别为3.52 mg/mL和2.75 mg/mL; 对胰脂肪酶的IC50为4.08 mg/mL, 与甘氨胆酸盐和牛磺胆酸盐的半数有效浓度(half maximal effective concentration, EC50)分别为4.45 mg/mL和 4.38 mg/mL。结论 超声辅助纤维素酶法可高效提取蜈蚣草多糖, 蜈蚣草多糖是一种潜在的降血糖、降血脂功能性食品原料。

英文摘要:

      Objective To investigate the extraction efficiency of Pteris vittata polysaccharides via an ultrasound-assisted cellulase method, and explore its hypoglycemic and hypolipidemic activities. Methods Response surface methodology was applied to optimize the technical parameters for the ultrasound-assisted cellulase extraction of Pteris vittata polysaccharides. The in vitro hypoglycemic activity was evaluated by determining the inhibition rates of α-amylase and α-glucosidase activities, while the in vitro hypolipidemic activity was assessed by analyzing the inhibition rate of pancreatic lipase activity as well as the binding rates to sodium glycocholate and sodium taurocholate. Results The optimal extraction parameters for Pteris vittata polysaccharides were determined as follows: solid-liquid ratio of 1:20 (g:mL), cellulase dosage of 2.2%, ultrasonic power of 316 W, ultrasonic temperature of 51 ℃ and ultrasonic time of 22 min. Under these conditions, the extraction yield of Pteris vittata polysaccharides was measured to be 4.19%±0.08%. In vitro bioactivity assessment demonstrated that Pteris vittata polysaccharides possessed certain hypoglycemic and hypolipidemic biological activities. The half-maximal inhibitory concentrations (IC50) of Pteris vittata polysaccharides against α-amylase and α-glucosidase were 3.52 mg/mL and 2.75 mg/mL, respectively. Its IC50 against pancreatic lipase was 4.08 mg/mL, and the half-maximal effective concentrations (EC50) for glycocholate and taurocholate were 4.45 mg/mL and 4.38 mg/mL, respectively. Conclusion The ultrasound-assisted cellulase method can efficiently extract Pteris vittata polysaccharides. Pteris vittata polysaccharides are potential functional food material with hypoglycemic and hypolipidemic properties.