响应面法优化酸枣仁肽-锌螯合物的制备工艺及结构表征研究

Optimization of preparation process and structural characterization of Ziziphi Spinosae Semen peptide-zinc chelates using response surface methodology

中文关键词:  酸枣仁  多肽  肽-锌螯合物  制备工艺  结构表征

英文关键词:Ziziphi Spinosae Semen  peptide  peptide-zinc chelate  preparation  structural characterization

中文摘要:

      目的 优化酸枣仁肽-锌螯合物(Ziziphi Spinosae Semen peptide-zinc chelate, ZSSP-Zn)的制备工艺, 并对其进行结构表征。方法 采用单因素结合响应面试验优化ZSSP-Zn的制备方法; 通过紫外光谱、红外光谱、氨基酸分析、扫描电镜及粒度分布对ZSSP-Zn的结构和组成进行表征; 通过体外模拟胃肠道消化研究ZSSP-Zn的稳定性。结果 ZSSP-Zn的最佳螯合条件为: 肽锌比3:1 (m:m), pH=6, 螯合时间2 h, 螯合温度60 ℃。在此条件下, 螯合率可达(41.97±0.82)%; 螯合后紫外吸收峰蓝移; 氨基氮原子、羧基氧原子、羰基氧原子、羟基氧原子是锌离子的重要结合位点; 螯合后呈疏松颗粒状, 粒度减小。体外模拟胃肠道消化分析结果表明, ZSSP-Zn稳定性较好。结论 本研究优化得到的ZSSP-Zn制备工艺稳定可靠, 螯合物粒度分布更为均一, 研究结果将为中药类锌补充剂的开发提供技术参考。

英文摘要:

      Objective To optimize the preparation process of Ziziphi Spinosae Semen peptide-zinc chelate (ZSSP-Zn) and characterize its structure. Methods This study optimized the preparation of ZSSP-Zn used a combination of single-factor experiments and response surface methodology. The structures and compositions of ZSSP-Zn were characterized using ultraviolet spectroscopy, Fourier transform infrared spectroscopy, amino acid analysis, scanning electron microscopy and size distribution. The stability of the ZSSP-Zn was investigated through in vitro simulated gastrointestinal digestion. Results The optimal chelation conditions for ZSSP-Zn included peptide-to-zinc ratio of 3:1 (m:m), pH 6, chelation time of 2 h and a chelation temperature of 60 ℃. Under these conditions, the chelation rate was (41.97±0.82)%. After chelation, the ultraviolet absorption peak shifts to the blue. Amino nitrogen atoms, carboxyl oxygen atoms, carbonyl oxygen atoms and hydroxyl oxygen atoms served as key binding sites for zinc ions. Chelation led to formation of loose granular structures with reduced particle size. The in vitro simulated gastrointestinal digestion analysis indicated that the ZSSP-Zn chelate exhibited good stability. Conclusion The optimized ZSSP-Zn preparation process in this study is stable and reliable, with a more uniform particle size distribution of the chelate complex. These results provide technical references for the development of traditional Chinese medicine-based zinc supplements.