磷虾油牡蛎软胶囊对绝经模型大鼠骨密度、骨代谢、炎症状态的综合干预研究

Comprehensive intervention of krill oil and oyster soft capsule on bone mineral density, bone metabolism and inflammatory state in postmenopausal rat models

中文关键词:  磷虾油  牡蛎  初乳碱性蛋白  去卵巢大鼠  骨密度  骨代谢

英文关键词:krill oil  oyster  colostrum basic protein  ovariectomized rats  bone mineral density  bone metabolism

中文摘要:

      目的 研究磷虾油牡蛎软胶囊对去卵巢大鼠骨密度、骨代谢及炎症状态的影响。方法 构建去卵巢大鼠模型, 分为假手术组、去卵巢对照组、磷虾油牡蛎软胶囊低、中、高剂量组和碳酸钙(CaCO3)组, 连续给样3个月。每周记录体重数据, 并在试验结束后测定大鼠体重变化、股骨骨钙含量、股骨中点和股骨远心端骨密度以及血清中骨保护素(osteoprotegerin, OPG)-核因子кB受体活化因子(receptor activator of nuclear factor kappa-B, RANK)-RANK配体(RANK ligand, RANKL)信号系统相关分子表达水平。结果 磷虾油牡蛎软胶囊低、中、高剂量能显著提高骨钙含量和股骨远心端骨密度, 中、高剂量能显著提高股骨中点骨密度, 且高于CaCO3处理组。骨代谢标志物检测结果显示, 磷虾油牡蛎软胶囊高剂量组明显提高碱性磷酸酶、骨钙素、I型前胶原氨基酸原肽表达水平, 低、中、高剂量组明显抑制骨碱性磷酸酶和I型胶原C末端肽表达水平。OPG-RANKL-RANK信号系统相关分子检测结果显示, 磷虾油牡蛎软胶囊低剂量组去卵巢大鼠血清中骨保护素水平显著下降; 中、高剂量组明显抑制去卵巢大鼠血清中骨保护素、白介素-6、白介素-8的水平, 并明显提高转化生长因子-β1的水平。结论 磷虾油牡蛎软胶囊能够通过提供钙源、维持骨形成和骨吸收的平衡、抗炎、调节OPG-RANKL-RANK信号系统及减少高骨转换率等多重机制, 改善去卵巢大鼠的骨密度、骨代谢及炎症状态。

英文摘要:

      Objective To study the effects of the krill oil and oyster soft capsules on bone mineral density, bone metabolism and inflammatory state in ovariectomized rats. Methods The ovariectomized rat models were established and divided into sham operation group, ovariectomized control group, low, medium and high dose of krill oil and oyster soft capsule groups and calcium carbonate group, which were continuously administered for 3 months. The body weight data were recorded weekly. At the end of the experiment, the changes in body weight, femoral bone calcium content, bone mineral density of the middle and distal femur, and the expression levels of osteoprotegerin (OPG)-receptor activator of nuclear factor kappa-B (RANK)-RANK ligand (RANKL) signaling system related molecules in serum were measured. Results The low, medium and high doses of krill oil and oyster soft capsules significantly increased femoral bone calcium content and bone mineral density of distal femur in ovariectomized rats; middle and high doses of krill oil and oyster soft capsules could significantly increase bone mineral density of middle femur, which was not lower than that of calcium carbonate treatment group. The results of bone metabolism markers showed that the expression levels of alkaline phosphatase, osteocalcin and procollagen type I amino acid propeptide significantly increased in the high dose group of kril oil and oyster soft capsules, while the expression levels of bone alkaline phosphatase and C-telopeptide of type I collagen were significantly inhibited in the low, medium and high dose groups. The results of OPG-RANKL-RANK signaling system related molecular detection showed that the low-dose kril oil and oyster soft capsules significantly inhibited the levels of osteoprotegerin, and the medium and high dose significantly inhibited the levels of osteoprotegerin, interleukin-6 and interleukin-8 in serum of ovariectomized rats, and significantly increased the level of transforming growth factor-β1. Conclusion The krill oil and oyster soft capsules can improve bone mineral density, bone metabolism and ameliorate inflammatory state in ovariectomized rats through multiple mechanisms such as providing calcium source, maintaining the balance between bone formation and bone resorption, anti-inflammation, regulating OPG-RANKL-RANK signaling system, and reducing high bone turnover rate.