固相萃取-超高效液相色谱法同时测定食品中单宁酸等9种食品添加剂

Simultaneous determination of 9 kinds of food additives including tannic acid in food samples by solid-phase extraction-ultra performance liquid chromatography

中文关键词:  单宁酸  甜味剂  防腐剂  固相萃取  超高效液相色谱法  食品添加剂

英文关键词:tannic acid  sweeters  preservatives  solid-phase extraction  ultra performance liquid chromatography  food additives

中文摘要:

      目的 建立超高效液相色谱法同时测定食品中单宁酸等9种食品添加剂的分析方法。方法 样品经水超声提取, PWA-2固相萃取柱净化后, 采用Poroshell 120 EC-C18型色谱柱(150 mm×3.0 mm, 2.7 μm)进行分离, 以25 mmol/L磷酸二氢钾和乙腈为流动相进行梯度洗脱, 在215 nm波长下检测。结果 9种目标物在2.0~100.0 mg/L质量浓度范围内线性关系良好, 相关系数(r)均不低于0.999; 方法检出限为0.0025~0.0050 g/kg, 定量限为0.005~0.010 g/kg。在茶饮料、肉肠、牛奶、红酒4种基质中进行低、中、高3个浓度水平的加标回收实验, 平均回收率为80.0%~118.0%, 相对标准偏差为2.8%~8.5% (n=6)。结论 该方法灵敏度高、重现性好、准确度高, 适用于食品中单宁酸等9种食品添加剂的同时检测。

英文摘要:

      Objective To establish a method for the simultaneous determination of 9 kinds of food additives in foods by ultra performance liquid chromatography. Methods The samples were subjected to ultrasonic extraction with water, purified by a PWA-2 solid-phase extraction cartridge, and then separated on a Poroshell 120 ECC18 column (150 mm×3.0 mm, 2.7 μm) under gradient elution using a mobile phase composed of 25 mmol/L potassium dihydrogen phosphate solution and acetonitrile. Detection was carried out at 215 nm. Results The results showed good linearity for the 9 kinds of analytes in the mass concentration range of 2.0–100.0 mg/L, with correlation coefficients (r2) not less than 0.999. The limits of detection and limits of quantitation ranged from 0.0025 to 0.0050 g/kg and from 0.005 to 0.010 g/kg, respectively. Recovery experiments were performed at low, medium and high spiked levels in 4 kinds of representative matrices (tea beverage, sausage, milk, red wine). The average recoveries were 80%–118%, with relative standard deviations in the range of 2.8%–8.5% (n=6). Conclusion The method exhibited high sensitivity, good reproducibility and high accuracy, and is applicable for the simultaneous determination of 9 kinds of food additives,including tannic acid, in food matrices.