高效液相色谱-串联质谱法检测保健食品中非法添加的喹诺酮类药物

Detection of illegally added quinolone drugs in health foods by high performance liquid chromatography-tandem mass spectrometry

中文关键词:  抗炎，保健食品  非法添加  喹诺酮类药物  高效液相色谱-串联质谱法

英文关键词:anti-inflammatory  health foods  illegal additives  quinolone drugs  high performance liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 建立高效液相色谱-串联质谱法检测抗炎保健食品中非法添加的4种喹诺酮类药物。方法 样品用乙腈溶解, 超声提取, 通过低温离心, 改良QuEChERS法净化, 用C18色谱柱分离后, 采用电喷雾离子源正离子模式下的多反应监测模式进行检测分析。结果 该方法对4种喹诺酮类药物线性良好, 相关系数(r)大于0.997, 检出限为0.01~0.02 mg/kg, 定量限为0.02~0.05 mg/kg, 回收率为70.16%~109.26%, 精密度为1.27%~4.56%。结论 该方法准确、简便、利于推广, 可实现抗炎保健食品中非法添加的喹诺酮类药物准确检测, 为保健食品中非法添加喹诺酮类药物的风险监测提供技术支撑。

英文摘要:

      Objective To establish a method for the detection of 4 kinds of quinolone drugs illegally added to anti-inflammatory health foods by high performance liquid chromatography-tandem mass spectrometry. Methods The sample was dissolved in acetonitrile, extracted by sonication, purified using a modified QuEChERS method with low-temperature centrifugation, separated using a C18 chromatographic column, and then analyzed using multiple reaction monitoring in positive ion mode with an electrospray ionization source. Results This method showed good linearity for 4 kinds of quinolone drugs, with a correlation coefficient (r) greater than 0.997. The limits of detection were 0.01–0.02 mg/kg, the limits of quantitation were 0.02–0.05 mg/kg, the recoveries rates were 70.16%–109.26% and the precisions were 1.27%–4.56%. Conclusion This method is accurate, simple and easy to implement, enabling accurate detection of quinolone drugs illegally added to anti-inflammatory health foods, and providing technical support for risk monitoring of illegally added quinolone drugs in health foods.