高效液相色谱法同时测定食品塑料包装产品中10种受限物质的迁移量

Simultaneous determination of migration levels of 10 kinds of restricted substances in food plastic packaging products by high performance liquid chromatography

中文关键词:  高效液相色谱法、食品塑料包装产品、受限物质

英文关键词:high performance liquid chromatography  food plastic packaging products  restricted substances

中文摘要:

      目的 建立高效液相色谱法同时测定食品塑料包装产品中包括双酚A、双酚S、双酚F、邻苯二甲酸、对苯二甲酸、间苯二甲酸、偏苯三甲酸、己内酰胺、1,1’-磺酰基二(4-氯苯)、4,4’-联苯二酚在内的10种受限物质迁移量的方法。方法 依据迁移实验通则对食品塑料包装产品进行迁移实验, 获得食品模拟液, 食品模拟液在色谱柱Welch Ultimate AQ-C18 (3.0 mm×100 mm, 3.5 μm)经0.1%磷酸水溶液-甲醇的流动相梯度洗脱分离, 二极管阵列检测器定量, 外标法定量。结果 在包括4%(体积分数)乙酸、10%(体积分数)乙醇、50%(体积分数)乙醇、95%(体积分数)乙醇的多种食品模拟物中, 10种化合物在0.10~10.00 mg/L定量范围内线性关系良好(r2>0.999), 检出限范围为0.01~0.02 mg/L, 定量限为0.05 mg/L, 低、中、高3个浓度加标水平下, 各类型食品模拟物的加标回收率为87.8%~104.0%, 相对标准偏差(n=6)为0.33%~7.58%。结论 该方法简便、高效、准确, 适用于食品塑料包装产品中10种受限物质的迁移量测定。

英文摘要:

      Objective To establish a method for the simultaneous determination of migration levels of 10 kinds of restricted substances [including bisphenol A, bisphenol S, bisphenol F, phthalic acid, terephthalic acid, isophthalic acid, trimellitic acid, caprolactam, 1,1’-sulfonylbis(4-chlorobenzene) and 4,4’-biphenol] in food plastic packaging products by high performance liquid chromatography. Methods Following the general principles of migration tests, plastic packaging products were subjected to migration testing using food simulants, the food simulants were separated by gradient elution with a mobile phase of 0.1% phosphoric acid aqueous solution-methanol on a Welch Ultimate AQ-C18 chromatographic column (3.0 mm×100 mm, 3.5 μm), detected by a diode array detector, and quantified by the external standard method. Results All 10 kinds of compounds exhibited good linear relationships in the concentration range of 0.10 to 10.00 mg/L (r2>0.999) in various food simulants, including 4% (volume fraction) acetic acid, 10% (volume fraction) ethanol, 50% (volume fraction) ethanol and 95% (volume fraction) ethanol. The limits of detection were in the range of 0.01–0.02 mg/L and the limits of quantitation were 0.05 mg/L. At 3 spiked concentration levels (low, medium and high), the recoveries of the target analytes in different food simulants ranged from 87.8% to 104.0%, with relative standard deviations (n=6) ranging from 0.33% to 7.58%. Conclusion This method is simple, efficient and accurate and is suitable for the determination of migration levels of 10 kinds of restricted substances in food plastic packaging products.