高产α-葡萄糖苷酶黑曲霉菌株的发酵条件优化

Optimization of fermentation conditions for Aspergillus niger strains with high-yield α-glucosidase

中文关键词:  α-葡萄糖苷酶,黑曲霉,发酵优化,正交试验

英文关键词:α-glucosidase  Aspergillus niger  fermentation optimization  orthogonal experiment

中文摘要:

      目的 优化高产α-葡萄糖苷酶黑曲霉菌株的发酵条件。方法 以黑曲霉为发酵菌种, 以α-葡萄糖苷酶酶活为评价指标, 利用单因素优化考察温度、转速、接种量等发酵条件, 应用正交试验优化发酵培养基成分。结果 黑曲霉发酵产α-葡萄糖苷酶最优发酵条件: 麦芽浸粉4%, 酵母蛋白胨3%, 曲拉通X-100 0.10%, K2HPO4·3H2O 0.5%, MgSO4·7H2O 0.05%, 初始发酵pH 5.9, 接种量2%。此条件下α-葡萄糖苷酶酶活为0.530 U/mL, 较优化前酶活提升28%。结论 本研究所优化得到的黑曲霉产α-葡萄糖苷酶发酵培养条件及发酵培养基成分, 与优化前相比, 显著地提高了α-葡萄糖苷酶酶活(P<0.05), 简便易行, 可为α-葡萄糖苷酶进一步工业化应用和产业化开发奠定理论基础。

英文摘要:

      Objective To optimize the fermentation conditions for Aspergillus niger strains with high-yield α-glucosidase. Methods Using Aspergillus niger as the fermentation strain and α-glucosidase activity as the evaluation index, single-factor optimization was employed to investigate fermentation conditions including temperature, rotation speed and inoculum size. Orthogonal experiment was applied to optimize the components of the fermentation medium. Results The optimal fermentation conditions for α-glucosidase production by Aspergillus niger were as follows: Malt extract powder 4%, yeast peptone 3%, Tween X-100 0.10%, K2HPO4·3H2O 0.5%, MgSO4·7H2O 0.05%, initial fermentation pH 5.9 and inoculum size 2%. Under these conditions, the α-glucosidase activity reached 0.530 U/mL, which was 28% higher than that before optimization. Conclusion The optimized fermentation conditions and medium components for α-glucosidase production by Aspergillus niger in this study significantly enhance the α-glucosidase activity compared with the unoptimized ones (P<0.05). The optimized strategy is simple and feasible, which lays a theoretical foundation for the further industrial application and commercial development of α-glucosidase.