黏质沙雷菌HBQT的生物信息挖掘及其特性分析

Bioinformatics mining and characteristic analysis of Serratia marcescens HBQT

中文关键词:  黏质沙雷菌  全基因组测序  生物信息学分析  亚硒酸盐  氧化应激

英文关键词:Serratia marcescens HBQT  whole genome sequencing  bioinformatics analysis  selenite  oxidative stress

中文摘要:

      目的 挖掘黏质沙雷菌HBQT的生物信息, 并分析其特性。方法 对黏质沙雷菌HBQT富硒能力进行评价并进行全基因组高通量测序及生物信息学分析, 测定其对金黄色葡萄球菌和单核增生李斯特氏菌的抑菌活性, 以及对绿豆芽的促生效果, 同时检测亚硒酸盐处理前后菌株对头孢他啶等药物的敏感性变化和生物膜生长情况以及抗氧化能力。结果 黏质沙雷菌HBQT 48 h内能够转化99%浓度为3 mmol/L的亚硒酸盐, 其基因组全长为5117438 bp, 鸟嘌呤和胞嘧啶含量59.51%, 含一个环状质粒; 生物信息学分析揭示其硫代谢与主动运输相关基因富集。该菌显著抑制金黄色葡萄球菌和单核增生李斯特氏菌生长(P<0.001), 并显著促进绿豆芽生长(P<0.001)。亚硒酸盐处理后, 菌株对头孢他啶的拮抗能力、生物膜形成能力及抗氧化力均显著提升(P<0.001)。结论 黏质沙雷氏菌HBQT具有较好的生物防治和植物促生特性, 并通过全基因组分析和其富硒后表型变化为细菌拮抗亚硒酸盐毒性生成纳米硒的分子机制阐明奠定基础。

英文摘要:

      Objective To mine the bioinformation of Serratia marcescens HBQT and analyze its characteristics. Methods The selenium enrichment capacity of Serratia marcescens HBQT was evaluated. High-throughput whole-genome sequencing and bioinformatics analysis were performed. Its antibacterial activity against Staphylococcus aureus and Listeria monocytogenes, as well as its growth-promoting effect on mung bean sprouts, were determined. Additionally, changes in the strain’s susceptibility to drugs such as ceftazidime, biofilm growth and antioxidant capacity were measured before and after selenite treatment. Results Within 48 hours, Serratia marcescens HBQT was able to convert 99% of the selenite at a concentration of 3 mmol/L. The complete genome length was 5117438 bp, with a guanine-cytosine content of 59.51%, and it contained one circular plasmid. Bioinformatics analysis revealed enrichment of genes related to sulfur metabolism and active transport. The strain significantly inhibited the growth of Staphylococcus aureus and Listeria monocytogenes (P<0.001) and markedly promoted the growth of mung bean sprouts (P<0.001). After selenite treatment, the strain’s antagonistic ability against ceftazidime, biofilm formation capacity and antioxidant capacity were significantly enhanced (P<0.001). Conclusion Serratia marcescens HBQT exhibits promising biocontrol and plant growth-promoting characteristics. The whole-genome analysis and phenotypic changes after selenium enrichment lay the foundation for elucidating the molecular mechanisms by which bacteria antagonize selenite toxicity and generate selenium nanoparticles.