胶体金免疫层析法快速检测鸡肉中尼卡巴嗪

Rapid detection of nicarbazine in chicken by colloidal gold immunochromatography

中文关键词:  尼卡巴嗪  4,4’-二硝基均二苯脲  胶体金免疫层析法  快速检测

英文关键词:nicarbazine  4,4’-dinitrocarbanilide  colloidal gold immunochromatographic  rapid detection

中文摘要:

      目的 建立胶体金免疫层析法快速检测鸡肉中尼卡巴嗪残留标志物4,4’-二硝基均二苯脲的方法。方法 通过对提取溶剂、用量、孵育时间等关键参数进行系统优化, 确定了最佳检测条件: 10.0 mL甲醇作为试样提取溶剂, 2 min提取时间, 孵育3 min。结果 实验验证结果显示: 对地克珠利、盐霉素等15种常见抗球虫药及5种结构类似物的交叉反应率均小于0.1%, 表明方法特异性强。在真实试样检测中与参比方法检测结果的一致性良好, 假阴性率和假阳性率为0%, 相对准确度100%。结论 本研究建立的方法操作简便、快速、灵敏、特异, 适用于鸡肉中尼卡巴嗪残留的现场快速筛查。

英文摘要:

      Objective To establish a method for the rapid detection of the nicarbazine residue marker 4,4’-dinitrogendibenzuron in chicken by colloidal gold immunochromatography. Methods Key parameters including extraction solvent, volume and incubation time were systematically optimized to determine the optimal detection conditions: 10.0 mL of methanol as the extraction solvent, an extraction time of 2 min and an incubation time of 3 min. Results Experimental validation demonstrated that the method exhibited cross-reactivity rates of less than 0.1% with 15 kinds of common anticoccidial drugs (e.g., diclazuril, salinomycin) and 5 kinds of structural analogs, indicating excellent specificity. When applied to real sample analysis, the results showed good agreement with those obtained by the reference method, with a false negative rate, false positive rate and relative accuracy of 0%, 0% and 100%, respectively. Conclusion The developed method is simple, rapid, sensitive and specific, making it suitable for on-site rapid screening of nicarbazin residues in chicken.