QuEChERS-气相色谱-三重四极杆质谱法同时测定食用植物油中10种农药残留量

Simultaneous determination of 10 kinds of pesticide residues in edible vegetable oils by QuEChERS-gas chromatography-triple quadrupole mass spectrometry

中文关键词:  QuEChERS法  气相色谱-三重四极杆质谱联用仪  食用植物油  农药残留

英文关键词:QuEChERS method  gas chromatography-triple quadrupole mass spectrometry  edible vegetable oils  pesticide residues

中文摘要:

      目的 建立了QuEChERS-气相色谱-三重四极杆质谱法同时测定食用植物油中10种农药残留的方法。方法 油样用乙腈提取, 经QuEChERS法净化后, 采用气相色谱-三重四极杆质谱联用仪检测, 以外标峰面积法定量。结果 10种农药在0.0025~0.5000 mg/L线性范围内均呈现良好的线性关系, 相关系数大于0.999, 本检测方法农药的定量限均为0.010 mg/kg, 方法验证试验对菜籽油、棕榈油和橄榄油进行了3个不同水平加标, 结果表明, 农药的平均回收率为85.0%~108.5%, 相对标准偏差均低于10%。结论 本方法准确度好, 精密度高, 操作简单快速, 适用于食用植物油中多种农药残留的检测。

英文摘要:

      Objective To develop a method for simultaneously determining 10 kinds of pesticide residues in edible vegetable oils by QuEChERS combined with gas chromatography-triple quadrupole mass spectrometry. Methods Oil samples were extracted with acetonitrile and purified using the QuEChERS method, followed by gas chromatography-triple quadrupole mass spectrometry detection and quantification using the external standard peak area method. Results The 10 kinds of pesticides showed good linear relationships within the range of 0.0025–0.5000 mg/L, with correlation coefficients greater than 0.999. The limit of quantitation of pesticides in this method was 0.010 mg/kg. Method validation tests involved 3 different levels of spiked samples in rapeseed oil, palm oil and olive oil. The results indicated that the average recoveries of pesticides ranged from 85.0% to 108.5%, with relative standard deviations all below 10%. Conclusion This method is accurate, precise, simple and fast, making it suitable for the detection of multiple pesticide residues in edible vegetable oils.