玉米粉中3种玉米赤霉烯酮真菌毒素的检测及其膳食暴露风险评估

Development of determination procedure and dietary exposure risk assessment for 3 kinds of zearalenone mycotoxins in maize flour

中文关键词:  玉米赤霉烯酮  ɑ-玉米赤霉烯醇  β-玉米赤霉烯醇  液相色谱-串联质谱法  QuEChERs,膳食暴露评估

英文关键词:zearalenone  ɑ-zearalenol  β-zearalenol  ultra performance liquid chromatography-tandem mass spectrometry  QuEChERS  dietary exposure risk assessment

中文摘要:

      目的 建立超高效液相色谱-串联质谱法同时检测玉米粉中玉米赤霉烯酮(zearalenone, ZEN)、ɑ-玉米赤霉烯醇(ɑ-zearalenol, ɑ-ZOL)、β-玉米赤霉烯醇(β-zearalenol, β-ZOL)含量的方法, 并对其人群暴露进行风险评估。方法 玉米粉样品经70%乙腈-水(V/V)振荡提取, QuEChERS-固相萃取净化, 超高效液相色谱-串联质谱法同时测定3种ZEN类毒素, 基质匹配外标法定量, 采用污染指数和风险商(hazard quotient, HQ)进行膳食暴露风险评估。结果 ZEN、ɑ-ZOL、β-ZOL分别在0.2~100.0、0.2~100.0、0.5~100.0 μg/L的范围内线性良好。ZEN、ɑ-ZOL和β-ZOL的检出限分别为0.5、0.5和1.5 μg/kg; 定量限分别为1.5、1.5和4.5 μg/kg。在5.0~60.0 μg/kg的添加浓度范围内, 3种目标物的回收率在80.4%~103.0%之间, 且精密度均小于15%。采用该方法检测30份市售玉米粉样品, ZEN检出率为80%, ɑ-ZOL和β-ZOL无检出。ZEN的污染指数小于0.7, HQ<1, 说明没有风险, 对人体健康威胁小。结论 该方法样品制备简单快速、准确、灵敏、能满足限量标准要求, 可用于玉米粉样品的检测, 有望进一步推广应用于粮食和粮食制品中3种ZEN类毒素的分析检测。 关键词: 玉米赤霉烯酮; ɑ-玉米赤霉烯醇; β-玉米赤霉烯醇; 超高效液相色谱-串联质谱法; QuEChERS; 膳食暴露风险评估

英文摘要:

      Objective To develop a method for the determination of zearalenone (ZEN), ɑ-zearalenol (ɑ-ZOL) and β-zearalenol (β-ZOL) in maize flour by ultra performance liquid chromatography-tandem mass spectrometry, and assess the dietary exposure risk. Methods The samples were extracted by 70% acetonitrile-water (V/V), purified by QuEChERS, then detected by ultra performance liquid chromatography-tandem mass spectrometry in multiple reaction monitoring mode and quantified by matrix matching curve. The pollution index and hazard quotient (HQ) are used for dietary exposure risk assessment. Results ZEN, ɑ-ZOL and β-ZOL showed good linearity within the ranges of 0.2–100.0, 0.2–100.0 and 0.5–100.0 μg/L respectively. The limits of detection of ZEN, ɑ-ZOL and β-ZOL were 0.5, 0.5 and 1.5 μg/kg, respectively; the limits of quantitation were 1.5, 1.5 and 4.5 μg/kg respectively. Within the addition concentration range of 5.0–60.0 μg/kg, the recovery rates of the 3 kinds of target substances were between 80.4% and 103.0%, and the precisions were all less than 15%. This method was used to test 30 commercially available maize flour samples. The detection rate of ZEN was 80%, while α-ZOL and β-ZOL were not detected. The pollution index of ZEN was less than 0.7 and the HQ of ZEN was less than 1, it indicated that there was no risk and the threat to human health was small. Conclusion The method is sensitive, accurate and effective for routine monitoring of ZEN, α-ZOL and β-ZOL in maize flour. This method can be expected to use for the determination of the ZEN, α-ZOL and β-ZOL in other grain and its products.