乳成分分析仪快速检测生乳营养成分

Rapid detection of raw milk nutritional components by milk composition analyzer

中文关键词:  生乳  快速检测  F检验  T检验

英文关键词:raw milk  rapid detection  F test  T test  Fourier infrared spectroscopy technology

中文摘要:

      目的 建立乳成分分析仪快速检测生乳营养成分的方法。方法 本研究以市售生乳为检测样品, 基于傅立叶红外光谱技术(Fourier transform infrared spectroscopy, FTIR)构建生乳中水分、蛋白质、脂肪、乳糖、灰分、非脂乳固体、总干物质、相对密度、冰点9个参数的快速检测方法, 通过与国家标准法结果比对评价快速检测方法的精确度和准确性。结果 乳成分分析仪测定结果为水分在86.00%~89.09%、蛋白质在2.52%~4.51%、脂肪在2.65%~5.39%、乳糖在3.81%~5.40%、灰分在0.56%~0.80%、非脂乳固体在6.94%~9.81%。通过对脂肪、乳糖、非脂乳固体等9个参数进行F检验及T检验, F值最大为9.00, 小于9.55, 证明9个参数精确性无显著性差异。T值最大为20.82, 小于22.327, 证明9个参数准确度不存在显著性差异。乳成分分析仪法及国家标准法均能对牛奶营养指标进行准确测定。显示所建立的快速检测方法的精确度和准确性与国家标准法无显著差异。结论 本研究所构建生乳营养成分快速检测方法可用于生乳营养成分的高通量、低成本的快速筛查。高精度场景、监管与准入时以国家标准法为基准, 日常监测以基于乳成分分析仪的快速检测结果为主。

英文摘要:

      Objective To establish a method for rapid detection of nutritional components in raw milk by milk composition analyzer. Methods This study used commercial raw milk as the test sample, and based on Fourier transform infrared spectroscopy (FTIR), it constructed a rapid detection method for 9 parameters such moisture, protein, fat, lactose, ash, non-fat milk solids, total dry matter, relative density and freezing point in raw milk. The precision and accuracy of the rapid detection method were evaluated by comparing it with the national standard detection results. Results The determination results of the milk composition analyzer were as follows: Moisture 86.00%–89.09%, protein 2.52%–4.51%, fat 2.65%–5.39%, lactose 3.81%–5.40%, ash 0.56%–0.80%, and non-fat milk solids 6.94%–9.81%. F-test and T-test were performed on 9 parameters including fat, lactose and non-fat milk solids. The maximum F-value was 9.00, less than 9.55, indicating no significant difference in precision for the 9 parameters. The maximum T-value was 20.82, less than 22.327, confirming no significant difference in accuracy for the 9 parameters. Both the milk composition analyzer method and the national standard method could accurately determine milk nutritional indicators. The results showed that the precision and accuracy of the established rapid detection method had no significant differences from those of the national standard method. Conclusion The rapid detection method for nutrient components in raw milk constructed in this study can be used for high-throughput and low-cost rapid screening of nutrient components in raw milk. National standard methods should be used as the benchmark for high-precision scenarios, supervision and admission, while daily monitoring should primarily rely on rapid detection results from milk composition analyzers.