碳纳米管-聚中性红电化学竞争性传感器检测小龙虾中腐胺

Detection of putrescine in Procambarus clarkii by carbon nanotubes-poly(neutral red) electrochemical competitive sensor

中文关键词:  小龙虾，腐胺，电化学传感器，鲜度，纳米材料

英文关键词:Procambarus clarkii  putrescine  electrochemical sensor  freshness  nanomaterial

中文摘要:

      目的 构建碳纳米管-聚中性红修饰电极用于小龙虾样品中腐胺的竞争性电化学快速检测。方法 以腐胺作为小龙虾鲜度特征物, 并针对腐胺设计了碳纳米管-聚中性红修饰电极, 探索电极电化学行为与腐胺浓度之间的关系, 并将其应用于腐胺的电化学传感研究。在优化了扫描速度、电解液pH等相关因素后, 测定小龙虾中的腐胺含量, 进行回收率实验, 并与高效液相色谱法进行对比。结果 水滑石-碳纳米管-聚中性红修饰电极对腐胺表现出明显的电化学抑制现象, 且腐胺的浓度与电极自身的峰电流对数值呈现出显著的反比关系, 其线性关系方程式为ΔI=3.8ln[c(PUT)]+8.3, 相关系数r=0.99。将其应用于小龙虾实际样品的腐胺检测, 该方法得到的腐胺含量与高效液相色谱法得到的结果高度一致。结论 该方法适合用于小龙虾中腐胺浓度的检测, 可以作为小龙虾鲜度特征物腐胺的生物传感器。

英文摘要:

      Objective To construct a carbon nanotube-poly(neutral red) modified electrode for the competitive electrochemical rapid detection of putrescine in Procambarus clarkii samples. Methods Putrescine was selected as a freshness indicator for Procambarus clarkii. A carbon nanotube-poly(neutral red) modified electrode was designed for putrescine detection. The relationship between the electrochemical behavior of the electrode and the concentration of putrescine was explored, and its application in electrochemical sensing of putrescine was investigated. After optimizing factors such as scan rate and electrolyte pH, the putrescine content in Procambarus clarkii was determined. Recovery experiments were conducted, and the results were compared with high performance liquid chromatography. Results The carbon nanotube-poly(neutral red) modified electrode exhibited a significant electrochemical inhibition effect towards putrescine, with a notable inverse relationship between the concentration of putrescine and the peak current of the electrode. The linear range was described by the equation ΔI=3.8ln[c(PUT)]+8.3, with a correlation coefficient of r=0.99. When applied to the detection of putrescine in crayfish samples, the results obtained by this method were highly consistent with those obtained by the high performance liquid chromatography. Conclusion The method can be employed for the detection of putrescine concentration in Procambarus clarkii and serves as a biosensor for putrescine, a freshness indicator of Procambarus clarkii.