基于MXene材料和X型DNA的荧光适配体传感器检测Pb2+和Cd2+

Fluorescent aptamer sensors based on MXene materials and X-type DNA for the detection of Pb2+ and Cd2+

中文关键词:  MXene纳米材料  X型DNA纳米结构  荧光生物传感器  重金属

英文关键词:MXene nanomaterials  X-type DNA nanostructures  fluorescent biosensors  heavy metals

中文摘要:

      目的 基于MXene纳米材料, 利用X型DNA和适配体构建了一种新型荧光适配体传感器, 用于同时检测食品中的Pb2+和Cd2+。方法 利用碱基互补配对制备X型DNA结构, 在Pb2+和Cd2+存在的情况下, X型DNA结构中的适配体与目标物特异性结合, 并触发MXene表面的解吸, 从而恢复荧光信号。结果 在最佳实验条件下, 荧光信号强度与Pb2+和Cd2+在0.5~500.0 nmol/L范围内呈线性相关, 检出限分别为0.33 nmol/L和0.28 nmol/L, 结果表明该传感器具有良好的工作性能。结论 本研究制备的荧光生物传感器已成功应用于自来水和麦片的加标分析, 具有良好的回收率, 表明可作为检测食品中Pb2+和Cd2+的有效工具。

英文摘要:

      Objective To construct a novel fluorescent aptamer sensor based on MXene nanomaterials by combining X-type DNA with aptamers for the simultaneous detection of Pb2+ and Cd2+ in food. Methods X-type DNA structures were prepared by complementary base pairing, and in the presence of Pb2+ and Cd2+, the aptamer within the X-type DNA structure specifically bound to the target ions and triggers desorption from the MXene surface, thereby restoring the fluorescent signal. Results Under the optimal experimental conditions, the fluorescence signal intensity was linearly correlated with Pb2+ and Cd2+ in the range of 0.5–500.0 nmol/L, with the limits of detection were 0.33 nmol/L and 0.28 nmol/L, respectively, and the results showed that the sensor had good working performance. Conclusion The fluorescent biosensor prepared in this study has been successfully applied to the spiked analysis of tap water and cornmeal with good recoveries, indicating that it can be used as an effective tool for the detection of Pb2+ and Cd2+ in food.