基于Ag3PO4纳米酶氧化活性快速比色检测鱼肉中组胺

Rapid colorimetric detection of histamine in fish based on the oxidase activity of Ag3PO4 nanozyme

中文关键词:  Ag3PO4  纳米酶  组胺  检测

英文关键词:Ag3PO4  nanozyme  histamine  fish

中文摘要:

      目的 建立基于Ag3PO4纳米酶氧化活性快速比色法检测鱼肉样品中组胺的方法。方法 通过水热法制备了Ag3PO4纳米颗粒, 并利用其氧化酶催化活性, 以CH3COONa为缓冲体系, 以3,3’,5,5’-四甲基联苯胺作为氧化底物, 建立了一种鱼肉中组胺的快速比色检测方法。结果 该方法检测时间在100 s左右, 检出限为0.58 mg/L, 具有较高的检测灵敏度; 在多种竞争性靶标检测中, 该方法表现出对组胺分子的良好的特异性; 在实际样品加标实验中, 该方法加标回收率为104.24%~108.30%, 相对标准偏差为1.81%~3.44%, 具有良好的检测准确性。结论 该方法表现出了直观、快速、灵敏度高、特异性好的优点, 能适应日常复杂的检测环境, 为鱼肉中组胺的检测提供了一种新的思路。

英文摘要:

      Objective To establish a rapid colorimetric method for the detection of histamine in fish samples based on the oxidase activity of Ag?PO? nanozyme. Methods Ag?PO? nanoparticles were prepared by the hydrothermal method. Utilizing their oxidase catalytic activity, with CH?COONa as the buffer system and 3,3’,5,5’-tetramethylbenzidine as the oxidation substrate, a rapid colorimetric detection method for histamine in fish was established. Results The detection time of this method was about 100 seconds. The limit of detection was 0.58 mg/L, indicating high detection sensitivity. In the detection of multiple competitive targets, this method showed good specificity for histamine molecules. In the spiked experiments on actual samples, the spiked recovery rate of this method ranged from 104.24% to 108.30%, and the relative standard deviations were from 1.81% to 3.44%, which had good detection accuracy. Conclusion This method demonstrates the advantages of being intuitive, rapid, highly sensitive and having good specificity. It can adapt to the complex daily detection environment and provides a new idea for the detection of histamine in fish meat.