不同月份舟山三叶青多成分含量测定及其指纹图谱的建立

Determination of multiple components content in Tetrastigma hemsleyanum Diels et Gilg at different months and establishment of its fingerprint chromatograms

中文关键词:  三叶青  不同季节  指纹图谱  含量测定  质量控制

英文关键词:Tetrastigma hemsleyanum Diels et Gilg  different seasons  fingerprint chromatograms  content determination  quality control

中文摘要:

      目的 探究舟山市不同月份三叶青主要活性成分并进行含量测定, 建立不同月份三叶青指纹图谱。方法 结合指纹图谱相似度评价、聚类分析(cluster analysis, CA)、主成分分析(principal component analysis, PCA)和正交偏最小二乘法判别分析(orthogonal partial least-squares discrimination analysis, OPLS-DA)进行不同月份舟山三叶青质量评价。采用InterSustainSwift C18色谱柱(4.6 mm×250 mm, 5 μm), 以乙腈-0.1%甲酸水溶液为流动相, 梯度洗脱, 流速0.8 mL/min, 检测波长320 nm, 柱温30 ℃, 进样量10 μL。结果 12批不同月份三叶青指纹图谱相似度评价结果表明, 不同月份三叶青样品的相似度差异较大; 共标定13个共有峰, 并确认3个共有峰; 通过CA、PCA和OPLS-DA分析均可将12批不同月份三叶青划分为3类, 且通过OPLS-DA项下的变量重要性投影(variable importance in the projection, VIP)值分析, 筛选出2个差异质量标志物, 分别是没食子酸和槲皮素。结论 该研究建立的高效液相色谱(high performance liquid chromatography, HPLC)指纹图谱和多成分含量测定方法稳定、可靠, 可为舟山三叶青的质量控制提供参考。

英文摘要:

      Objective To investigate the main active components of different monthly Tetrastigma hemsleyanum Diels et Gilg (TDG) from Zhoushan City and establish the map of different monthly TDG. Methods The quality of different monthly TDG was evaluated by combining fingerprint similarity evaluation cluster analysis (CA), principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). An InterSustainSwift C18 chromatographic column (4.6 mm×250 mm, 5 μm) was used, with acetonitrile-0.1 formic acid aqueous solution as the mobile phase, gradient elution, flow rate of 0.8 mL/min, detection wavelength of 20 nm, column temperature of 30 ℃, and injection volume of 10 μL. Results The fingerprint similarity evaluation results of 12 batches of different monthly TDG showed that the similarity of different monthly TDG samples varied greatly; the 13 common were identified, and 3 common peaks were recognized; CA, PCA, and OPLS-DA analysis could all classify the 12 batches of different monthly TDG into 3 categories; and 2 differential quality markers, gallic acid and quercetin, were screened out by analyzing the variable importance in projection (VIP) values under OPLS-DA. Conclusion The high performance liquid chromatography (HPLC) fingerprint and multi-component content determination method established in this study is stable and reliable, and can provide a reference for quality control of Zhoushan TDG.