土壤杆菌A02分批发酵产可得然胶动力学模型的建立

Establishment of kinetic model for the batch fermentation production of curdlan by Agrobacterium sp. A02

中文关键词:  土壤杆菌  可得然胶  发酵动力学模型  分批发酵

英文关键词:Agrobacterium sp.  curdlan  fermentation kinetic model  batch fermentation

中文摘要:

      目的 预测和控制土壤杆菌A02发酵产可得然胶的过程, 建立随时间变化的模型, 模拟细菌的生长, 可得然胶生成以及蔗糖消耗的动力学变化。方法 对土壤杆菌分批发酵过程中的菌体浓度、蔗糖含量、可得然胶产量进行测定, 分别采用Logistic方程、Luedeking-Piret方程和Luedeking-Piret-Like方程对实验值进行拟合。结果 拟合结果表明构建的3种模型适用于描述菌体生长动力学、可得然胶生成动力学和蔗糖消耗动力学, 其R2均在0.99以上, 显著性水平均为极显著。模型拟合值与实验值误差均小于10%, 拟合良好。结论 建立的菌体生长动力学模型、蔗糖消耗动力学模型和可得然胶生成动力学模型都能较好地描述和预测土壤杆菌A02在分批发酵产可得然胶过程中的动态代谢变化, 为描述发酵动力学特征和可得然胶工业化生产提供了理论支持。

英文摘要:

      Objective To predict and control the fermentation process of Agrobacterium sp. A02 strain to produce curdlan, establish a time-varying model to simulate bacterial growth and obtain the kinetic changes of curdlan production and sucrose consumption. Methods The viable bacteria count, sucrose content and curdlan content during the batch fermentation process of Agrobacterium sp. A02 were measured, and the experimental values were fitted using Logistic equation, Luedeking-Piret equation and Luedeking-Piret-Like equation, respectively. Results The fitting results indicated that the 3 kinds of models were applicable to the growth kinetics of bacterial cells, the generation kinetics of pectin, and the consumption kinetics of sucrose. The R2 were above 0.99, and the significance level was extremely significant. The errors between the fitted and experimental values were less than 10%, indicating a good fit. Conclusion The established bacterial growth kinetics model, sucrose consumption kinetics model and curdlan production kinetics model can predict and describe the dynamic metabolic changes of Agrobacterium sp. A02 during the fermentation process, providing theoretical support for describing the fermentation kinetics characteristics and industrial production of curdlan.