核桃多肽-硒螯合物的制备及其理化性质研究

Preparation and physicochemical properties research of Juglans regia L. polypeptide-selenium chelate

中文关键词:  核桃多肽-硒螯合物，理化性质，稳定性，体外消化，抗氧化能力

英文关键词:Juglans regia L. polypeptide-selenium chelate  physicochemical properties  stability  in vitro digestion  antioxidant capacity

中文摘要:

      目的 探究核桃多肽-硒螯合物的制备方法及其理化性质。方法 采用碱溶酸沉法提取核桃蛋白, 经碱性蛋白酶酶解后制得核桃多肽。核桃多肽再与亚硒酸钠反应, 生成核桃多肽-硒螯合物。通过单因素试验和正交试验, 优化制备工艺。结果 在多肽与亚硒酸钠质量比为3:1、螯合温度45 ℃、螯合时间50 min、pH 8的条件下, 硒螯合率达到最高, 为58.99%±1.44%。该螯合物耐热、耐酸碱, 消化稳定性好, 硒生物利用率高。与核桃多肽相比, 核桃多肽-硒螯合物具有更强的1,1-二苯基-2-苦味酰肼(1,1-diphenyl-2-picrylhydrazyl, DPPH)自由基、羟基自由基和2,2’-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐[2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), ABTS]阳离子自由基清除能力。结论 本研究制备了核桃多肽-硒螯合物, 优化了制备工艺, 并发现其具有良好的理化性质和抗氧化能力, 为开发补硒产品提供了原料参考。

英文摘要:

      Objective To study the preparation and physicochemical properties of polypeptide-selenium chelate from Juglans regia L.. Methods Juglans regia L. protein was extracted by alkali-soluble acid precipitation method, and Juglans regia L. polypeptide was prepared by alkaline protease. Juglans regia L. polypeptide reacted with sodium selenite to produce Juglans regia L. polypeptide-selenium chelate. The preparation technology was optimized by single factor test and orthogonal test. Results When the mass ratio of polypeptide to sodium selenite was 3:1, the chelating temperature was 45 ℃, the chelating time was 50 min, and the pH was 8, the chelating rate was 58.99%±1.44%. The chelate was resistant to heat, acid and alkali, had good digestive stability and high bioavailability of selenium. Compared with Juglans regia L. polypeptide, Juglans regia L. polypeptide-selenium chelate had stronger scavenging ability of 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical, hydroxyl free radical and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation free radical. Conclusion In this study, Juglans regia L. polypeptide-selenium chelate is successfully prepared through optimized synthesis conditions. The resulting complex demonstrates favorable physicochemical properties and significant antioxidant capacity, providing a potential raw material reference for developing selenium-enriched nutritional supplements.