计时电流法检测食品中亚硝酸盐的含量

Determination of nitrite content in food by chronoamperometry

中文关键词:  钴纳米粒子  氧化石墨烯  亚硝酸钠  电化学

英文关键词:cobalt nanoparticles  grapheme oxide  nitrite  electrochemistry  chronoamperometry

中文摘要:

      目的 建立计时电流法精确测定食品中亚硝酸盐含量。方法 将钴纳米粒子、氧化石墨烯以及聚吡咯聚沉于电极表面, 构建检验亚硝酸根的电化学传感器, 对检测条件进行优化, 确定了计时电流法检测亚硝酸盐的线性范围, 对检测方法的特异性进行验证后利用优化后的检测条件对样品进行检测。结果 制备出一种检测亚硝酸盐的电化学传感器, 其最优电位确定为0.85 V, 最优pH为8.0, 最优沉积时间为300 s, 干扰物质多巴胺、抗坏血酸、尿酸对于亚硝酸盐的检测结果影响较小, 且对样品进行检测发现, 所建立的方法准确度较高。结论 制备出检验亚硝酸根的电化学传感器, 该系统稳定性好、精确度高, 适用于食品中亚硝酸盐含量的测定。

英文摘要:

      Objective To establish a chronoamperometry detection method for accurately determining the nitrite content in food. Methods Cobalt nanoparticles, graphene oxide, and polypyrrole were electrodeposited on the electrode surface to construct an electrochemical sensor for nitrite detecting. The detection conditions were optimized, and the linear range of chronocurrent method for nitrite detection was determined. After the specificity of the detection method was verified, the samples were tested using the optimized detection conditions. Results An electrochemical sensor for nitrite detection was prepared. The optimal potential for this method was 0.85 V, the optimal pH was 8.0, and the optimal deposition time was 300 s. The interfering substances dopamine, ascorbic acid and uric acid had little impact on the detection of nitrite, and the detection of samples found that the established method had high accuracy. Conclusion An electrochemical sensor for detecting nitrite is prepared, which has good stability and high accuracy, and is suitable for the determination of nitrite content food.