干、鲜铁皮石斛对正常小鼠肠黏膜菌群的影响

Effects of dried and fresh Dendrobium officinale on the mucosa-associated microbiota of normal mice

中文关键词:  铁皮石斛  鲜品  干品  肠道菌群  小肠  结肠

英文关键词:Dendrobium officinale  gut microbiota  small intestine  colon

中文摘要:

      目的 探究铁皮石斛干品及鲜品对正常小鼠肠道菌群的影响。方法 分别给正常小鼠灌胃铁皮石斛干品及鲜品7 d, 观察小鼠的生长情况, 用16S rRNA基因全长序列测序技术检测小肠及结肠黏膜菌群的变化。结果 铁皮石斛干品与鲜品均可控制小鼠体重增长, 但鲜品的作用更显著。二者均降低了结肠黏膜菌群的α多样性, 但鲜品提高了小肠黏膜菌群的α多样性, 干品降低了小肠黏膜菌群的α多样性。铁皮石斛鲜品显著促进小肠黏膜中Lactobacillus及Faecalibacterium prausnitzii、Butyricicoccus pullicaecorum、Roseburia inulinivorans等短链脂肪酸产生菌的增殖, 上调了脂质代谢、萜类及聚酮类代谢、碳水化合物代谢、外源物质生物降解与代谢等通路; 在结肠黏膜中, 铁皮石斛干品抑制Escherichia coli及Ruminococcus gnavus等肠道疾病相关细菌的作用强于鲜品。结论 铁皮石斛鲜品可通过促进有益菌增殖, 上调脂质及碳水化合物代谢以控制体重, 维持机体的健康状态; 而铁皮石斛干品可抑制有害菌增长, 或有助于降低潜在的疾病风险。

英文摘要:

      Objective To explore the effects of dried Dendrobium officinale and fresh Dendrobium officinale on the gut microbiota of normal mice and to provide a reference for its rational development and application. Methods The normal mice were administrated with dried Dendrobium officinale and fresh Dendrobium officinale for 7 days, respectively. The growth of mice was observed, and the mucosa-associated microbiota in the small intestine and colon was detected using the full-length 16S rRNA gene sequencing. Results The results showed that both dried Dendrobium officinale and fresh Dendrobium officinale could control the weight gain of mice, but the weight-control effect of fresh Dendrobium officinale was more significant than that of dried Dendrobium officinale. Both dried Dendrobium officinale and fresh Dendrobium officinale decreased α diversity of mucosa-associated microbiota in the colon. However, fresh Dendrobium officinale increased α diversity of mucosa-associated microbiota in the small intestine, while the effect of dried Dendrobium officinale was the opposite. Moreover, fresh Dendrobium officinale significantly promoted the proliferation of short-chain fatty acid-producing bacteria (Lactobacillus, Faecalibacterium prausnitzii, Butyricicoccus pullicaecorum and Roseburia inulinivorans) in the small intestine and up-regulated metabolic pathways such as lipid metabolism, metabolism of terpenoids and polyketides, carbohydrate metabolism, and xenobiotics biodegradation and metabolism. In colonic mucosa-associated microbiota, dried Dendrobium officinale showed a stronger inhibitory effect on Escherichia coli and Ruminococcus gnavus than fresh Dendrobium officinale. Conclusion The results show that fresh Dendrobium officinale can control body weight and maintain health by promoting the proliferation of beneficial bacteria and up-regulating lipid and carbohydrate metabolism, while dried Dendrobium officinale can inhibit the growth of harmful bacteria, and may help reduce the potential risk of disease.