2021—2022年上海市区零售鸡肉和猪肉馅中沙门氏菌血清型和耐药特征研究

Study on serotypes and drug resistance characteristics of Salmonella in retail chicken and pig meat fillings in Shanghai in 2021—2022

中文关键词:  沙门氏菌  血清型  耐药性  食源性疾病

英文关键词:Salmonella  serotype  drug resistance  foodborne disease

中文摘要:

      目的 了解上海市区零售鸡肉和猪肉馅中沙门氏菌分离株的血清型分布和耐药性特征。方法 收集2021—2022年上海市区零售鸡肉和猪肉馅中分离的109株沙门氏菌, 使用血清凝集方法进行血清学鉴定, 采用微量肉汤稀释法, 对7类14种抗菌药物进行药物敏感检测, 采用全基因组测序分析其耐药基因。结果 109株沙门氏菌共检出22种血清群, 鸡肉源沙门氏菌以科瓦利斯血清型为主, 猪肉源沙门氏菌以伦敦血清型为主。96.3%的菌株为耐药株, 菌株对四环素、氨苄西林、氯霉素和复方磺胺耐药率最高, 多重耐药菌株占全部菌株的61.4%, 最高可对7类12种抗菌药物耐药。所有菌株均检出耐药基因, 共得到68种耐药基因。结论 2021—2022年上海零售生禽畜肉中沙门氏菌的血清型分布较为复杂, 整体耐药水平较高, 多重耐药性严重, 携带多种耐药基因, 耐药性与携带的耐药基因存在相关性。对食源性沙门氏菌的持续监测以及针对重要耐药和传播机制开展深入研究, 对保障我国食品安全和指导临床一线用药有重大的意义。

英文摘要:

      Objective To investigate the serotype distribution and antibiotic resistance characteristics of Salmonella isolated from retail poultry and pork meat in Shanghai. Methods The 109 Salmonella strains isolates from retail chicken meat and pork meat in Shanghai in 2021—2022 were collected, and serotyped by serum agglutination method. The antibiotic susceptibility of 7 classes of 14 kinds of antibacterial drugs was determined by microbroth dilution method, and the antibiotic resistance genes were analyzed by whole genome sequencing. Results A total of 22 serotypes were identified in the 109 Salmonella strains, with the Corvallis serotype being the predominant one in retail poultry chicken meat and the London serotype being the predominant one in pork meat. The 96.3% of the isolates strains were resistant to antibiotics, with the highest resistance rates to tetracycline, ampicillin, chloromycetin, and cotrimoxazole. Multi-drug resistant isolates accounted for 61.4% of all isolates, with the highest resistance to 7 classes of 12 antibiotics. All isolates were found to harbor resistance genes, with a total of 68 resistance genes identified. Conclusion The serotype of Salmonella isolated from retail poultry and pork meat in Shanghai in 2021—2022 is complex, with a high overall level of resistance and severe multi-resistance. The isolates carry multiple resistance genes, and the drug resistance is correlated with the resistance genes they carried. Continuous monitoring of foodborne Salmonella and in-depth research on important resistance mechanisms and transmission mechanisms are of great significance for ensuring food safety and guiding clinical drug use in China.