低共熔溶剂液-液微萃取-高效液相色谱法检测辣椒粉中6种苏丹红色素含量

Determination of 6 kinds of Sudan dyes in Capsicum annuum powder by deep eutectic solvent liquid-liquid microextraction-high performance liquid chromatography

中文关键词:  低共熔溶剂  液-液微萃取  高效液相色谱法  苏丹红  辣椒粉

英文关键词:deep eutectic solvent  liquid-liquid microextraction  high performance liquid chromatography  Sudan dye  Capsicum annuum powder

中文摘要:

      目的 建立一种基于低共熔溶剂(deep eutectic solvent, DES)萃取结合高效液相色谱法快速检测辣椒粉中6种苏丹红的方法。方法 利用液-液微萃取方法提取辣椒粉中的苏丹红Ⅰ、苏丹红Ⅱ、苏丹红Ⅲ、苏丹红Ⅳ、苏丹红7B、苏丹红G。将提取液通过微孔滤膜过滤后进行高效液相色谱法测定, 外标法定量。考察DES稀释倍数、DES摩尔比、DES用量、萃取时间和萃取方式对6种苏丹红色素萃取效果的影响。结果 最优方法条件为DES稀释倍数为5倍、DES摩尔比1:2.5、DES用量600 μL、萃取时间50 s、萃取方式为涡旋萃取, 在此条件下, 建立的方程在质量浓度0.1~50.0 mg/L范围内, 线性关系良好, 相关系数均大于0.999, 检出限为0.03~0.20 mg/L, 定量限为0.10~1.00 mg/L。其中苏丹红G、苏丹红Ⅰ和苏丹红Ⅱ的回收率较高, 为71.6%~117.5%, 相对标准偏差为0.6%~4.7%。结论 本研究建立的方法操作简便、高效、绿色环保, 可对辣椒粉中6种苏丹红进行快速检测。

英文摘要:

      Objective To establish an analytical method for the determination of 6 kinds of Sudan dyes in Capsicum annuum powder based on liquid-liquid microextraction with deep eutectic solvents (DES) combined with high performance liquid chromatography. Methods Sudan red I, Sudan red II, Sudan red III, Sudan red IV, Sudan red 7B and Sudan red G in Capsicum annuum powder were extracted by liquid-liquid microextraction. The extract was filtered through a microporous membrane and then determined by high performance liquid chromatography with external standard method for quantification. The effects of DES dilution ratio, DES molar ratio, DES addition amount, extraction time, and extraction method on the extraction efficiency of 6 kinds of Sudan dyes were investigated. Results The results showed that the optimal method conditions were: Dilution ratio of DES 5 times, molar ratio of DES 1:2.5, addition amount of DES 600 μL, extraction time 50 s, and extraction method was vortex extraction. Under these conditions, the established equation had a good linear relationship in the range of mass concentration 0.1–50.0 mg/L, the correlation coefficients were all greater than 0.999, the limits of detection were 0.03–0.20 mg/kg, and the limits of quantitative were 0.10–1.00 mg/kg. The recovery rates of Sudan red G, Sudan red I, and Sudan red II were higher, ranging from 71.6% to 117.5%, with relative standard deviations of 0.6% to 4.7%. Conclusion The established method is simple, efficient, and environmentally friendly, and can be used for rapid detection of 6 kinds of Sudan dyes in Capsicum annuum.