林仁权,夏海鸣,戚荣平,孟 琪,桑 娴.微乳液相色谱法测定酱油中乙二胺四乙酸铁钠含量[J].食品安全质量检测学报,2025,16(5):269-275 |
微乳液相色谱法测定酱油中乙二胺四乙酸铁钠含量 |
Determination of ethylenediaminetetraacetic acid ferric sodium salt content in soy sauce by microemulsion liquid chromatography |
投稿时间:2024-06-17 修订日期:2024-07-31 |
DOI: |
中文关键词: 乙二胺四乙酸铁钠 微乳液相色谱法 十六烷基三甲基溴化铵 酱油 |
英文关键词:ethylenediaminetetraacetic acid ferric sodium salt microemulsion liquid chromatography cetyltrimethylammonium bromide soy sauce |
基金项目:杭州市拱墅区医药卫生科技计划项目A类项目A202308 |
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中文摘要: |
目的 建立微乳液相色谱法测定酱油中乙二胺四乙酸铁钠(ethylenediaminetetraacetic acid ferric sodium salt, NaFeEDTA)含量的分析方法。方法 样品经简单的稀释和离心, 通过0.22 μm微孔滤膜进行过滤, 即可直接上机, 以Waters BEH C18柱(2.1 mm×150 mm, 1.7 μm)为色谱柱, 以3.64 g/L十六烷基三甲基溴化铵水溶液-50 g/L乙腈-8 g/L乙酸乙酯-6.8 g/L磷酸二氢钾水溶液为流动相, 流速为0.30 mL/min, 检测波长为254 nm, 柱温为30 ℃, 使用外标法进行定量测定。结果 在选定实验条件下, NaFeEDTA质量浓度在0.50~35.00 μg/mL范围内具有良好的线性关系(r≥0.999), 检出限为50 mg/kg。考察了NaFeEDTA回收率, 其平均回收率分别为92.7%~97.7%。结论 该方法操作简单、重现性好, 可用于酱油中NaFeEDTA含量的检测。 |
英文摘要: |
Objective To establish a method for the determination of ethylenediaminetetraacetic acid ferric sodium salt (NaFeEDTA) by microemulsion liquid chromatography. Methods After simple dilution and centrifugation of the sample, it could be filtered through a 0.22 μm microporous membrane and directly loaded onto a Waters BEH C18 column (2.1 mm×150 mm, 1.7 μm) was the chromatographic column. The microemulsion mobile phase was 3.64 g/L cetyltrimethylammonium bromide-50 g/L acetonitrile-8 g/L ethyl acetate-6.8 g/L potassium dihydrogen phosphate. The flow rate was 0.30 mL/min and the detection wavelength was 254 nm. The column temperature was 30 ℃ temperature. External standard method was used for quantitative determination. Results Under the selected experimental conditions, the mass concentration of NaFeEDTA had a good linear relationship (r≥0.999) within the range of 0.50–35.00 μg/mL, the limits of detection were 50 mg/kg. The recovery rates of NaFeEDTA were investigated, with average recovery rates ranging from 92.7% to 97.7%. Conclusion This method is easy to operate and has good reproducibility. The method can be used for the detection of NaFeEDTA content in soy sauce. |
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