林仁权,夏海鸣,戚荣平,孟 琪,桑 娴.微乳液相色谱法测定酱油中乙二胺四乙酸铁钠含量[J].食品安全质量检测学报,2025,16(5):269-275
微乳液相色谱法测定酱油中乙二胺四乙酸铁钠含量
Determination of ethylenediaminetetraacetic acid ferric sodium salt content in soy sauce by microemulsion liquid chromatography
投稿时间:2024-06-17  修订日期:2024-07-31
DOI:
中文关键词:  乙二胺四乙酸铁钠  微乳液相色谱法  十六烷基三甲基溴化铵  酱油
英文关键词:ethylenediaminetetraacetic acid ferric sodium salt  microemulsion liquid chromatography  cetyltrimethylammonium bromide  soy sauce
基金项目:杭州市拱墅区医药卫生科技计划项目A类项目A202308
作者单位
林仁权 1.杭州市拱墅区疾病预防控制中心(杭州市拱墅区卫生监督所) 
夏海鸣 1.杭州市拱墅区疾病预防控制中心(杭州市拱墅区卫生监督所) 
戚荣平 1.杭州市拱墅区疾病预防控制中心(杭州市拱墅区卫生监督所) 
孟 琪 1.杭州市拱墅区疾病预防控制中心(杭州市拱墅区卫生监督所) 
桑 娴 1.杭州市拱墅区疾病预防控制中心(杭州市拱墅区卫生监督所) 
AuthorInstitution
LIN Ren-Quan 1.Gongshu Center for Disease Prevention and Control (Gongshu Health Supervision Institution) 
XIA Hai-Ming 1.Gongshu Center for Disease Prevention and Control (Gongshu Health Supervision Institution) 
QI Rong-Ping 1.Gongshu Center for Disease Prevention and Control (Gongshu Health Supervision Institution) 
MENG Qi 1.Gongshu Center for Disease Prevention and Control (Gongshu Health Supervision Institution) 
SANG Xian 1.Gongshu Center for Disease Prevention and Control (Gongshu Health Supervision Institution) 
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中文摘要:
      目的 建立微乳液相色谱法测定酱油中乙二胺四乙酸铁钠(ethylenediaminetetraacetic acid ferric sodium salt, NaFeEDTA)含量的分析方法。方法 样品经简单的稀释和离心, 通过0.22 μm微孔滤膜进行过滤, 即可直接上机, 以Waters BEH C18柱(2.1 mm×150 mm, 1.7 μm)为色谱柱, 以3.64 g/L十六烷基三甲基溴化铵水溶液-50 g/L乙腈-8 g/L乙酸乙酯-6.8 g/L磷酸二氢钾水溶液为流动相, 流速为0.30 mL/min, 检测波长为254 nm, 柱温为30 ℃, 使用外标法进行定量测定。结果 在选定实验条件下, NaFeEDTA质量浓度在0.50~35.00 μg/mL范围内具有良好的线性关系(r≥0.999), 检出限为50 mg/kg。考察了NaFeEDTA回收率, 其平均回收率分别为92.7%~97.7%。结论 该方法操作简单、重现性好, 可用于酱油中NaFeEDTA含量的检测。
英文摘要:
      Objective To establish a method for the determination of ethylenediaminetetraacetic acid ferric sodium salt (NaFeEDTA) by microemulsion liquid chromatography. Methods After simple dilution and centrifugation of the sample, it could be filtered through a 0.22 μm microporous membrane and directly loaded onto a Waters BEH C18 column (2.1 mm×150 mm, 1.7 μm) was the chromatographic column. The microemulsion mobile phase was 3.64 g/L cetyltrimethylammonium bromide-50 g/L acetonitrile-8 g/L ethyl acetate-6.8 g/L potassium dihydrogen phosphate. The flow rate was 0.30 mL/min and the detection wavelength was 254 nm. The column temperature was 30 ℃ temperature. External standard method was used for quantitative determination. Results Under the selected experimental conditions, the mass concentration of NaFeEDTA had a good linear relationship (r≥0.999) within the range of 0.50–35.00 μg/mL, the limits of detection were 50 mg/kg. The recovery rates of NaFeEDTA were investigated, with average recovery rates ranging from 92.7% to 97.7%. Conclusion This method is easy to operate and has good reproducibility. The method can be used for the detection of NaFeEDTA content in soy sauce.
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