微乳液相色谱法测定酱油中乙二胺四乙酸铁钠含量

Determination of ethylenediaminetetraacetic acid ferric sodium salt content in soy sauce by microemulsion liquid chromatography

中文关键词:  乙二胺四乙酸铁钠  微乳液相色谱法  十六烷基三甲基溴化铵  酱油

英文关键词:ethylenediaminetetraacetic acid ferric sodium salt  microemulsion liquid chromatography  cetyltrimethylammonium bromide  soy sauce

中文摘要:

      目的 建立微乳液相色谱法测定酱油中乙二胺四乙酸铁钠(ethylenediaminetetraacetic acid ferric sodium salt, NaFeEDTA)含量的分析方法。方法 样品经简单的稀释和离心, 通过0.22 μm微孔滤膜进行过滤, 即可直接上机, 以Waters BEH C18柱(2.1 mm×150 mm, 1.7 μm)为色谱柱, 以3.64 g/L十六烷基三甲基溴化铵水溶液-50 g/L乙腈-8 g/L乙酸乙酯-6.8 g/L磷酸二氢钾水溶液为流动相, 流速为0.30 mL/min, 检测波长为254 nm, 柱温为30 ℃, 使用外标法进行定量测定。结果 在选定实验条件下, NaFeEDTA质量浓度在0.50~35.00 μg/mL范围内具有良好的线性关系(r≥0.999), 检出限为50 mg/kg。考察了NaFeEDTA回收率, 其平均回收率分别为92.7%~97.7%。结论 该方法操作简单、重现性好, 可用于酱油中NaFeEDTA含量的检测。

英文摘要:

      Objective To establish a method for the determination of ethylenediaminetetraacetic acid ferric sodium salt (NaFeEDTA) by microemulsion liquid chromatography. Methods After simple dilution and centrifugation of the sample, it could be filtered through a 0.22 μm microporous membrane and directly loaded onto a Waters BEH C18 column (2.1 mm×150 mm, 1.7 μm) was the chromatographic column. The microemulsion mobile phase was 3.64 g/L cetyltrimethylammonium bromide-50 g/L acetonitrile-8 g/L ethyl acetate-6.8 g/L potassium dihydrogen phosphate. The flow rate was 0.30 mL/min and the detection wavelength was 254 nm. The column temperature was 30 ℃ temperature. External standard method was used for quantitative determination. Results Under the selected experimental conditions, the mass concentration of NaFeEDTA had a good linear relationship (r≥0.999) within the range of 0.50–35.00 μg/mL, the limits of detection were 50 mg/kg. The recovery rates of NaFeEDTA were investigated, with average recovery rates ranging from 92.7% to 97.7%. Conclusion This method is easy to operate and has good reproducibility. The method can be used for the detection of NaFeEDTA content in soy sauce.