高效液相色谱法测定蜂王浆和蜂王浆干粉中羟甲基糠醛含量

Determination of hydroxymethylfurfural in royal jelly and its dry powder by high performance liquid chromatography

中文关键词:  蜂王浆  蜂王浆干粉  羟甲基糠醛  液相色谱法  新鲜度

英文关键词:royal jelly  royal jelly dry powder  hydroxymethylfurfural  high performance liquid chromatography  freshness

中文摘要:

      目的 建立高效液相色谱法测定蜂王浆和蜂王浆干粉中羟甲基糠醛含量。方法 将蜂王浆经氨水碱化, 使样液呈中性或者弱碱性, 用乙酸乙酯提取, 离心, 取上清液蒸干后复溶解, 过0.22 μm滤膜, 采用C18色谱柱分离, 以羟甲基糠醛为标准品进行外标法定量, 以高效液相色谱仪进行检测。结果 羟甲基糠醛在20~500 μg/kg范围内线性良好, 相关系数r2为0.9983, 添加回收率为86.1%~100.8%, 相对标准偏差为4.6%~8.0%, 检出限为6 μg/kg, 定量限为20 μg/kg。对11个样品进行测定, 蜂王浆和蜂王浆干粉中羟甲基糠醛含量不等, 与储存条件有关。结论 本研究方法可用于检测蜂王浆及其冻干粉中羟甲基糠醛的含量, 羟甲基糠醛具有评价蜂王浆及其冻干粉新鲜度的潜力。

英文摘要:

      Objective To establish a method for determination of hydroxymethylfurfural in royal jelly and its dry powder by high performance liquid chromatography. Methods Royal jelly was alkalized with ammonia to make the sample neutral or weakly alkaline. The royal jelly sample was extracted using ethyl acetate and then centrifuged. The supernatant was evaporated to dryness and then dissolved. After passed through 0.22 μm filter membrane, the resultant sample was separated by C18 column and tested using liquid chromatography. The sample was quantified by external standard method as the standard of hydroxymethylfurfural. Results Hydroxymethylfurfural had good linearity in the range of 20–500 μg/kg. The correlation coefficients were 0.9983. The recoveries of addition were 86.1%–100.8%, and the relative standard deviation were 4.6%–8.0%. The limit of detection was 6 μg/kg and limit of quantitation was 20 μg/kg. The contents of hydroxymethylfurfural in 11 samples royal jelly and royal jelly dry powder were different and related to storage conditions. Conclusion This method can be used to determine the content of hydroxymethylfurfural in royal jelly and its freeze-dried powder, and hydroxymethylfurfural has the potential to evaluate the freshness of royal jelly and its freeze-dried powder.