吉林地区奶源金黄色葡萄球菌耐药性及遗传特征分析

Analysis of antibiotic resistance and genetic characteristics of milk derived Staphylococcus aureus in Jilin

中文关键词:  乳汁  金黄色葡萄球菌  耐药性  遗传特征  全基因组测序

英文关键词:fresh milk  Staphylococcus aureus  drug resistance  genetic characteristics  whole genome sequencing

中文摘要:

      目的 了解鲜牛乳中金黄色葡萄球菌(Staphylococcus aureus, S. aureus)流行情况及耐药情况、分子特征和遗传特征。方法 细菌分离培养及聚合酶链式反应(polymerase chain reaction, PCR)鉴定S. aureus, K-B药敏纸片扩散法检测其耐药性, 对其中44个分离株进行全基因组测序(whole genomic sequence, WGS), 并分析分子特征及遗传特性等。结果 共分离出214株S. aureus, 对13种常用抗生素均有耐药性, 对青霉素产生耐药性菌株最多(145/214, 67.76%)。44个流行株中, 耐药表型达31种, 25个菌株有多重耐药; 检测到β-内酰胺类、四环素类等12类耐药基因及季铵盐类消毒剂耐药基因, 其中6株检测到耐利奈唑胺基因; L8株携带11种毒力基因, 至少90%的流行株携带aur、hlgA/B/C基因毒力; ST9-t899是流行优势型。SNP-WGS全基因组遗传进化分析, 提示有人、畜间互传可能性。结论 吉林地区牛场样本中S. aureus流行率为42.80%, 存在多重耐药, 且耐药谱广、耐药表型多样; 有人畜间传播可能。鲜牛乳作为初级食品, 对其中S. aureus特别是耐甲氧西林金黄色葡萄球菌耐药性及分子特征监测, 是对食品卫生及公共卫生安全的重要保障。

英文摘要:

      Objective To explore the prevalence, antimicrobial resistance phenotype and genotype, molecular and genetic characteristics of Staphylococcus aureus (S. aureus) in fresh milk. Methods Bacterial isolation and polymerase chain reaction (PCR) were used to identify S. aureus, K-B drug sensitive disk diffusion were used to detect drug resistance of isolated strains, then 44 isolated strains were used for whole genome sequencing to analyze their molecular and genetic characteristics. Results A total of 214 strains of S. aureus were isolated, which were resistant to 13 kinds of common antibiotics, and the most resistant strains were penicillin (145/214, 67.76%). Among the 44 endemic strains, 31 types of drug-resistant phenotypes were found, and 25 strains had multiple drug resistance. The 12 kinds of drug resistance genes such as β-lactam, tetracycline and quaternary ammonium salt disinfectant were detected, and linezolid resistance genes were detected in 6 strains. L8 strain carried with 11 virulence genes, and at least 90% of strains carried aur and hlgA/B/C virulence genes; ST9-t899 was a dominant type. SNPs-WGS suggested the possibility of transmission between humans and animals. Conclusion The prevalence of S. aureus in cattle farm in Jilin is 42.80%, with multiple drug resistance, wide drug resistance spectrum and diverse drug resistance phenotype; and human-to-animal transmission is possible. As a primary food, monitoring the antimicrobial resistance and molecular characteristics of S. aureus, especially methicillin-resistant S. aureus, in fresh milk is an important guarantee for food hygiene and public health safety.