海鱼中海洋神经毒素污染特征及暴露风险评估

Pollution characteristics and exposure risk assessment of marine neurotoxins in marine fish

中文关键词:  海洋毒素  海鱼  超高效液相色谱-高分辨质谱  风险评估

英文关键词:marine toxins  sea fish  ultra performance liquid chromatography-tandem mass spectrometry  risk assessment

中文摘要:

      目的 调查我国南海区域典型海洋神经毒素主要污染的海鱼品种及污染水平, 为污染分布、暴露评估提供技术手段。方法 采用固相萃取结合超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry, UPLC-MS/MS)对海鱼样品中的石房蛤毒素(saxitoxin, STX)、新石房蛤毒素(neosaxitoxin, NEO)、膝沟藻毒素(gonyautoxin, GTX-1、GTX-4、GTX-5)、N-磺酰氨甲酰基类毒素(N-sulfocarbamoyltoxins, C1、C2)、脱氨甲酰石房蛤毒素(decarbamoylsaxitox, dcSTX)、脱氧脱氨甲酰基新石房蛤毒素(decarbamoylneosaxitoxin, dcNEO)、脱氧脱氨甲酰基膝沟藻毒素(decarbamoylgonyautoxin-2&3, dcGTX 2、dcGTX 3)、河豚毒素(tetrodotoxins, TTX) 12种海洋神经毒素进行测定, 外标法定量。结果 在1.2~50.0 μg/kg浓度范围内, 12种海洋神经毒素呈良好的线性关系(R2≥0.9990), 48种海鱼样本中, 有13种检出海洋神经毒素, 各省份平均暴露水平均在安全范围内, 提示了潜在的急性暴露风险。结论 建立了海产品中多种海洋神经毒素的UPLC-MS/MS痕量检测技术并将其应用于南海海域的毒素污染特征及暴露风险评估, 为海鱼中海洋神经毒素的检测和控制提供了技术支撑和理论依据。

英文摘要:

      Objective To investigate the species and pollution levels of typical marine neurotoxins in the South China Sea, and provide technical means for pollution distribution and exposure assessment. Methods Solid phase extraction combined with ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was used to detect saxitoxin (STX), neosaxitoxin (NEO), gonyautoxin (GTX-1, GTX-4, GTX-5), N-sulfocarbamoyltoxins (C1, C2), decarbamoylsaxitox (dcSTX), decarbamoylneosaxitoxin (dcNEO), decarbamoylgonyautoxin-2&3 (dcGTX 2, dcGTX 3), tetrodotoxins (TTX) 12 kinds of marine neurotoxins were determined. The samples were quantified by external standard method. Results In the concentration range of 1.2?50.0 μg/kg, 12 kinds of marine neurotoxins showed a good linear relationship (R2≥0.9990). Among 48 kinds of marine fish samples, 13 kinds of marine neurotoxins were detected, and the average exposure levels in all provinces were within the safe range, suggesting the potential acute exposure risk. Conclusion The UPLC-MS/MS trace detection technology for various marine neurotoxins in seafood is established and applied to the assessment of toxin contamination characteristics and exposure risk in the South China Sea, which provide technical support and theoretical basis for the detection and control of marine neurotoxins in marine fish.