基于静电作用诱导金铂纳米粒子聚集的比色方法检测鱼肉中组胺

Colorimetric detection of histamine in fish based on electrostatic interaction induced gold platinum nanoparticles aggregation

中文关键词:  金铂纳米粒子  比色法  组胺  生物胺

英文关键词:gold platinum nanoparticles  colorimetric method  histamine  biogenic amine

中文摘要:

      目的 基于金铂纳米粒子(AuPt nanoparticles, AuPt NPs)建立一种简便、灵敏的比色方法检测鱼肉中的组胺。方法 由柠檬酸钠制备的AuPt NPs表面带负电, 组胺通过静电作用能与AuPt NPs相结合, 诱导AuPt NPs聚集, 溶液由棕红色变为灰色; 同时引起AuPt NPs表面等离子体共振发生红移, 吸收峰由510 nm移至650 nm附近, 将650 nm和510 nm的吸光度比值(A650/A510)作为检测组胺的参数。结果 在最优的条件下(反应时间为1.0 h、反应温度为25℃), A650/A510随着组胺浓度的升高而升高, A650/A510与0.5~30.0 μmol/L的组胺浓度呈现良好的线性关系, 线性方程为A650/A510=0.019C组胺+0.3686 (r2=0.9962), 方法检出限为0.32 μmol/L。将方法用于鱼肉样品的检测, 加标回收率为102.1%~111.7%, 相对标准偏差为1.8%~3.1%。结论 本实验方法具有简便、快捷和成本低的优点, 可实现鱼肉中组胺的可视化快速检测。

英文摘要:

      Objective To establish a simple and sensitive colorimetric method to detect histamine in fish based on AuPt nanoparticles (AuPt NPs). Methods AuPt NPs prepared from sodium citrate which was negatively charged on the surface, combined with histamine by electrostatic interaction, thus caused a red shift of the plasma resonance on the surface of AuPt NPs and induced the aggregation of AuPt NPs, and the solution changed from brownish-red to gray. The absorption peak was shifted from 510 nm to near 650 nm. The absorbance ratio between 650 nm and 510 nm (A650/A510) was used as a standard parameter for the detection of histamine. Results Under the optimal experimental conditions (reaction time of 1.0 h and reaction temperature of 25℃), the A650/A510 ratio increased with the increase histamine concentration, and showed a good linear relationship in the range of 0.5–30.0 μmol/L, and the corresponding equation was A650/A510=0.019Chistamine+0.3686 (r2=0.9962). The limit of detection was 0.32 μmol/L. The method was applied to the detection of fish samples, and the recovery rate was 102.1%-111.7%, and the relative standard deviation was 1.8%-3.1%.Conclusion This experimental method has the advantages of simplicity, rapidity and low cost, and can realize the visual rapid detection of histamine in fish.