不同抑菌剂对枯草芽孢杆菌芽孢动态生长的影响

Effects of different bacteriostats on the dynamic germination of Bacillus subtilis spores

中文关键词:  枯草芽孢杆菌  抑菌剂  芽孢  萌发  抑制

英文关键词:Bacillus subtilis  bacteriostats  spores  germination  inhibit

中文摘要:

      目的 研究不同抑菌剂对枯草芽孢杆菌(Bacillus subtilis)芽孢生长的影响及作用阶段。方法 本研究选取枯草芽孢杆菌芽孢为研究对象, 考查了茶多酚、Nisin、亚硝酸盐、双乙酸钠、乙二胺四乙酸盐5种抑菌剂对枯草芽孢杆菌芽孢存活量、萌发特性、内容物泄漏量、结构变化的影响。结果 5种抑菌剂均能降低枯草芽孢杆菌芽孢的存活率, 且不同抑菌剂对枯草芽孢杆菌芽孢生长的主要抑制阶段存在差异。其中乙二胺四乙酸盐、茶多酚在芽孢萌发阶段的作用效果相对较为显著, 与芽孢悬浊液共孵育60 min后吸光度分别下降至90.61%、90.74%, 2,6-吡啶二羧酸释放量分别为21.18%、21.92%, 孵育12 h后仍有部分芽孢保持“相亮”状态。亚硝酸盐、双乙酸钠、Nisin主要在萌发后发挥抑制作用, 其中亚硝酸盐、双乙酸钠能使芽孢在后期生长过程中内膜通透性增大, 并导致芽孢核内物质发生泄漏。拉曼光谱显示茶多酚、亚硝酸盐能够使得芽孢生长过程中蛋白质、脂质等成分及其结构发生变化, 使萌发后的芽孢无法继续生长形成营养体。结论 乙二胺四乙酸盐、茶多酚在芽孢萌发阶段发挥主要作用, 亚硝酸盐、双乙酸钠、Nisin在萌发后及营养体形成阶段发挥主要抑制作用。本研究为揭示不同抑菌剂对芽孢生长的抑菌机制提供重要信息和参考。

英文摘要:

      Objective To study the effects of different bacteriostatic agents on the growth of Bacillus subtilis spores and their stages of action. Methods In this paper, Bacillus subtilis spores were selected as the research object, and the effects of tea polyphenols, Nisin, nitrite, sodium diacetate and ethylenediamine tetraacetate on the survival, germination characteristics, content leakage and structural changes of Bacillus subtilis spores were studied. Results Five kinds of bacteriostatic agents could reduce the survival rates of Bacillus subtilis spores, and the main inhibition stages of different bacteriostatic agents on the growth of Bacillus subtilis spores were different. Among them, ethylenediamine tetraacetate and tea polyphenols played a relatively significant role in the germination stage of spores. After incubation with spore suspension for 60 min, the absorbance decreased by 90.61% and 90.74%, respectively, and the release of dipicolinic acid were 21.18% and 21.92%, respectively. After incubation for 12 h, some spores remained “bright”. Nitrite, sodium diacetate and Nisin mainly played an inhibitory role after germination, among which nitrite and sodium diacetate could increase the permeability of the inner membrane of spores in the later growth process, and lead to the leakage of materials in the spores. Raman spectra showed that tea polyphenols and nitrites could lead to changes in the surface properties of spores, protein, lipids and other components and their structures, so that germinated spores could not continue to grow to form vegetative bodies. Conclusion Ethylenediamine tetraacetate and tea polyphenols play a major role in the germination stage of spores, while nitrite, sodium diacetate and Nisin play a major inhibitory role after germination and vegetative formation stage. This study provides important information and references for revealing the bacteriostatic mechanism of different bacteriostatic agents on spore growth.