中国沙棘雄花花被和花粉蛋白质营养评价

Protein nutrition evaluation of the tepals and pollen of Hippophae rhamnoides L. subsp. sinensis Rousi male flower

中文关键词:  中国沙棘  雄花  花粉  蛋白质  营养评价

英文关键词:Hippophae rhamnoides L. subsp. sinensis Rousi  male flower  pollen  protein  nutritional evaluation

中文摘要:

      目的 分析中国沙棘雄花花被及花粉蛋白质含量和氨基酸组成及其营养均衡性。方法 采用凯氏定氮法和柱后衍生阳离子交换色谱方法分析中国沙棘雄花花被及花粉中蛋白质和氨基酸的含量, 并结合国际上通用的营养价值评价方法对其蛋白质含量、化学评分(chemical sore, CS)、氨基酸评分(aminoacid score, AAS)、必需氨基酸指数(essential amino acid index, EAAI)、氨基酸比值系数分(score of ratio coefficient of amino acid, SRCAA)、生物价(biological value, BV)和营养指数(nutritional index, NI)等进行综合评价。结果 中国沙棘雄花花被及花粉蛋白质含量分别为16.07%、20.45%; 中国沙棘雄花花被及花粉中氨基酸含量无显著差异, 17种氨基酸总含量分别为13.59 g/100 g、17.06 g/100 g; 蛋白质营养评价表明, 中国沙棘雄花花被及花粉中总必需氨基酸分别为31.14 g/100 g、33.10 g/100 g, 接近于联合国粮农组织与世界卫生组织(Food and Agriculture Organization of the United Nations/World Health Organization, FAO/WHO)模式(35.0 g/100 g)而低于全鸡蛋模式(49.7 g/100 g)。CS和AAS显示, 含硫氨基酸(蛋氨酸+半胱氨酸)为中国沙棘雄花花被及花粉的第一限制性氨基酸; 两个样品氨基酸比值系数分别为99.51、98.07, 均接近于FAO/WHO模式氨基酸分值(100), 营养价值高; 必需氨基酸指数为52.80、61.20。结论 中国沙棘雄花花被及花粉氨基酸含量丰富, 种类较齐全, 可作为理想的蛋白质来源, 并与其他日常膳食搭配作为氨基酸补充剂应用。

英文摘要:

      Objective To analyze the protein content, amino acid composition and nutritional balance of the tepals and pollen of Hippophae rhamnoides L. subsp. sinensis Rousi male flowers. Methods Used a method of Keldahl nitrogen determination and cation-exchange column method for the analysis of protein and amino acids in the tepals and pollen of male flowers of Hippophae rhamnoides L. subsp. sinensis Rousi. The protein content, chemical sore (CS), aminoacid score (AAS), essential amino acid index (EAAI), score of ratio of amino acids (SRCAA), biological value (BV) and nutritional index (NI) and amino acid nutritional balance were measured by the international common nutrional evaluation method. Results The content of protein in tepals and pollen of Hippophae rhamnoides L. subsp. sinensis Rousi was 16.07% and 20.45%, respectively. There was no significant difference in tepals and pollen of Hippophae rhamnoides L. subsp. sinensis Rousi. The total content of 17 kinds of amino acids were 13.59 g/100 g and 17.06 g/100 g. Protein nutrition evaluation showed that the total essential amino acid in the tepals and pollen of Hippophae rhamnoides L. subsp. sinensis Rousi was 31.14 g/100 g and 33.10 g/100 g, which was close to the Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO) model (35.0 g/100 g) but lower than the whole egg model (49.7 g/100 g). CS and AAS showed that sulfur amino acid (methionine+cysteine) was the first limiting amino acid in the male tepals and pollen of Hippophae rhamnoides L. subsp. sinensis Rousi. The amino acid ratio coefficients of the two samples were 99.51 and 98.07, respectively, which were close to the amino acid score of FAO/WHO model (100), indicating high nutritional value. The essential amino acid index were 52.80 and 61.20, respectively. Conclusion The tepals and pollen of Hippophae rhamnoides L. subsp. sinensis Rousi are rich in amino acids, which can be used as an ideal source of protein, and can be used as amino acid supplement with other daily diets.