红托竹荪菌托黄酮的纯化及其抗氧化与抗疲劳活性研究

Study on purification, anti-oxidation and anti-fatigue activity of flavonoids from Dictyophora rubrovolvata

中文关键词:  红托竹荪菌托  黄酮  纯化工艺  抗氧化  抗疲劳

英文关键词:Dictyophora rubrovolvata  flavonoids  purification process  anti-oxidation: anti-fatigue activity

中文摘要:

      目的 研究红托竹荪菌托黄酮的纯化工艺, 及其抗氧化与抗疲劳活性。方法 采用大孔树脂法纯化红托竹荪菌托黄酮。以树脂吸附率和解吸率为评价指标, 对红托竹荪菌托黄酮的纯化工艺参数进行优化, 并研究红托竹荪菌托黄酮纯化前后对1,1-二苯基-2-苦基肼(1,1-diphenyl-2-bitter hydrazine, DPPH)自由基和羟基(OH)自由基清除能力的影响和对小鼠的抗疲劳效果。结果 红托竹荪菌托黄酮的最佳纯化工艺条件为: 吸附条件: 上样液质量浓度1.2 mg/mL、上样液流速1.5 mL/min、上样液pH为5, 上样液体积60 mL; 解吸条件: 乙醇浓度70%、洗脱流速1.5 mL/min、洗脱液体积120 mL, 经D101型大孔树脂纯化后, 红托竹荪菌托黄酮提取物中总黄酮的纯度由14.16%提高到57.64%, 对DPPH自由基和OH自由基的半数抑制浓度分别为0.16和0.78 mg/mL; 与粗提物相比, 纯化后的黄酮提取物可明显延长小鼠的负重游泳时间, 减少其运动后血清中血乳酸和血尿素氮的含量。结论 红托竹荪菌托黄酮经纯化后具有较好的抗氧化性和抗疲劳活性, 对红托竹荪菌托黄酮资源的开发利用提供理论依据。

英文摘要:

      Objective To study the purification process of flavonoids from Dictyophora rubrovolvata, and their antioxidant and anti-fatigue activities. Methods The flavonoids from Dictyophora rubrovolvata were purified by macroporous resin method. Based on the resin adsorption rate and desorption rate as evaluation indexes, the purification process parameters of flavonoids from Dictyophora rubrovolvata were optimized, the effects of flavonoids from Dictyophora rubrovolvata before and after purification on the scavenging capacity of 1,1-diphenyl-2-bitter hydrazine (DPPH) radical and OH radical were investigated and the anti-fatigue effects on mice were also studied. Results The optimum purification parameters of flavonoids from Dictyophora rubrovolvata were as follows: Adsorption conditions: The sample mass concentration was 1.2 mg/mL, the flow rate of the sample was 1.5 mL/min, the pH of the sample solution was 5, the volume of the sample solution was 60 mL; desorption conditions: The concentration of ethanol was 70%, the flow rate of the elution solution was 1.5 mL/min, the volume of the elution solution was 120 mL. After purification with D101 macroporous resin, the purity of total flavones in the extract from Dictyophora rubrovolvata was increased from 14.16% to 57.64%. The half inhibitory concentration of DPPH radical and OH radical were 0.16 and 0.78 mg/mL, respectively. Compared with the crude extract, the purified extract could obviously prolong the weight-bearing swimming time and decrease the content of blood lactic acid and blood urea nitrogen in post-exercise serum of mice. Conclusion The purified flavonoids from Dictyophora rubrovolvata have good anti-oxidation and anti-fatigue activities, which provide a theoretical basis for the development and utilization of the flavonoids from Dictyophora rubrovolvata.