表面增强拉曼光谱法快速检测百合中的地虫硫磷

Rapid detection of fonofos in Lilium brownii var. viridulum Baker by surface enhanced Raman spectroscopy

中文关键词:  L-半胱氨酸  金纳米粒子  表面增强拉曼光谱法  百合  地虫硫磷

英文关键词:L-cysteine  Au nanoparticles  surface enhanced Raman spectroscopy  Lilium brownii var. viridulum Baker  fonofos

中文摘要:

      目的 制备具有良好增强效果的L-半胱氨酸功能化修饰的金纳米粒子(L-cysteine-Au nanoparticles, L-cys-AuNPs), 建立表面增强拉曼光谱法(surface-enhanced Raman spectroscopy, SERS)快速检测百合中地虫硫磷的分析方法。方法 采用柠檬酸钠还原法制备AuNPs, 然后用L-半胱氨酸进行功能化修饰。通过单因素实验对增强基底添加量、地虫磷添加量、凝聚剂种类等实验条件进行优化, 以确定最佳实验条件。结果 该基底表现出优异的增强效果, 1071 cm?1可作为其特征峰。地虫硫磷的检出限为0.02 mg/L, 线性范围是0.02~0.50 mg/L, 相关系数r2=0.9667, 回收率为69%~112%, 相对标准偏差(relative standard deviations, RSDs)为7%~8%。结论 该方法灵敏度高、特异性、稳定性较好, 可用于百合中地虫硫磷的快速检测。

英文摘要:

      Objective To prepare L-cysteine functionalized gold nanoparticles (L-cysteine-Au nanoparticles, L-cys-AuNPs) with good enhancement effect, and establish a method for the rapid detection of fonofos in Lilium brownii var. viridulum Baker by surface-enhanced Raman spectroscopy (SERS). Methods AuNPs were prepared by sodium citrate reduction and then functionalized with L-cysteine. Then the single factor experiment was conducted to optimize the experimental conditions, such as the amount of enhanced substrate, the amount of fonofos and the type of coagulant, so as to determine the best experimental conditions. Results The substrate showed excellent enhancement effect, and 1071 cm?1 could be used as its characteristic peak. The limit of detection was 0.02 mg/L, the linear range was 0.02?0.50 mg/L, the correlation coefficient (r2) was 0.9667, the recoveries were 69%?112%, and the relative standard deviations (RSDs) were 7%?8%. Conclusion The method is sensitive, specific and stable, and can be used for the rapid detection of fonofos in Lilium brownii var. viridulum Baker.