适配体传感器在玉米赤霉烯酮检测中的应用

Application of aptasensors in the detection of zearalenone

中文关键词:  玉米赤霉烯酮  适配体  传感器

英文关键词:zearalenone  aptamer  sensor

中文摘要:

      玉米赤霉烯酮(zearalenone, ZEN)是由镰刀菌属产生的一种非甾体真菌毒素, 广泛存在于霉变的玉米、高粱等谷类作物以及奶制品中。由于其具有类雌激素作用, 对动物和人均具有潜在的危害。因此, 开发灵敏快速的ZEN检测技术对于防控ZEN具有重要意义。近年来, 适配体传感器(aptasensors)因其分子量小、亲和力强、易于合成和修饰及稳定性好等优点, 已广泛应用于临床诊断、药物分析、环境监测、食品安全等领域。尤其在食品安全检测领域, 适配体传感器可提供灵活多变的构建策略, 且易于实现现场快速检测。因此本文结合国内外的研究进展, 综述了近些年来基于适配体传感器的玉米赤霉烯酮检测方法, 概括了各种检测方法的原理及其在实际样品检测中的应用, 旨在为发展新的分析检测方法提供参考。

英文摘要:

      Zearalenone (ZEN) is a non-steroidal mycotoxin produced by Fusarium, which is widely found in mildew corn, sorghum and other cereal crops as well as dairy products. Because of its estrogen-like effect, ZEN poses a potential threat to animal and human health.??Hence, it is of great significance to develop sensitive and rapid technology to prevent and control ZEN. In recent years, because aptamers own various advantages including small molecular weight, high affinity to the target, easy synthesis and convenient modification, and good stability, aptamer-based sensors (aptasensors), have been intensively applied in clinical diagnosis, drug analysis, environmental monitoring, food safety and so on. Especially in the field of food safety, aptasensors can provide flexible construction strategies, and easy to achieve rapid on-site testing. Therefore, based on the recent research progress at home and abroad, this paper reviewed different aptasensors for ZEN detection, and summarized the principles of aptasensors and their applications in actual samples, aiming to provide a reference for the development of new analytical methods.