紫薯腐败菌的分离与鉴定

Isolation and identification of spoilage bacteria from Solanum tuberdsm

中文关键词:  紫薯  分离  鉴定  腐败菌

英文关键词:Solanum tuberdsm  isolation  identification  spoilage bacteria

中文摘要:

      目的 从自然腐败的紫薯上分离纯化紫薯腐败菌, 通过反接种确定其致腐性, 并将其鉴定到属。方法 通过多次稀释涂布平板法进行单菌落形态学观察, 将菌株反接种到新鲜紫薯上, 筛选出腐败菌, 以真菌ITS序列、细菌16S rDNA序列构建发育树, 鉴定到属, 再将鉴定结果与分离的菌株的形态学鉴定结果对比, 验证菌株鉴定结果。结果 从样品中分离出6种典型腐败菌, 有4种属, 分别为枝孢霉属、青霉属、曲霉属、芽孢杆菌属。结论 确定紫薯的腐败菌为哥氏枝孢霉(Cladosporium gossypiicola)、基督青霉(Penicillium christenseniae)、热带青霉(Penicillium tropicum)、黄曲霉(Aspergillus flavus)、贝莱斯芽孢杆菌(Bacillus velezensis)和阿氏芽孢杆菌(Bacillus aryabhattai)。

英文摘要:

      Objective To isolate and purify the spoilage bacteria from natural spoilage Solanum tuberdsm, then determine its spoilage by anti-inoculation, and then identify the genus. Methods The morphology of single colony was observed by multiple dilution coating plate method. Then the strains were inoculated on fresh Solanum tuberdsm, and the spoilage bacteria were screened out. The phylogenetic tree was constructed with fungal sequence and bacterial 16S rDNA sequence, and the genus was identified. The identification results were compared with the morphological identification results of isolated strains to verify the identification results. Results Six kinds of typical spoilage bacteria were isolated from the samples, including 4 kinds of genera: Cladosporium, Penicillium, Aspergillus and Bacillus. Conclusion Cladosporium gossypiicola, Penicillium christensenae, Penicillium tropicum, Aspergillus flavus, Bacillus velezensis and Bacillus aryabhattai are identified as the spoilage bacteria of Solanum tuberdsm.