干酪乳杆菌SB27降低N-二甲基亚硝胺对大鼠肠黏膜细胞损伤作用研究

Study on Lactobacillus casei SB27 reducing the damage of N-nltrosodimethylamine to intestinal mucosal cells in rats

中文关键词:  干酪乳杆菌SB27  N-二甲基亚硝胺  IEC-6细胞  细胞损伤

英文关键词:Lactobacillus casei SB27  N-nltrosodimethylamine  IEC-6 cells  cell damage

中文摘要:

      目的 研究干酪乳杆菌SB27 (Lactobacillus casei SB27)降低N-二甲基亚硝胺(N-nltrosodimethylamine, NDMA)对大鼠肠道黏膜细胞IEC-6毒性作用的能力。方法 通过CCK-8实验、苏木精-伊红(hematoxylin-eosin, HE)染色、TUNEL染色和透射电镜对干酪乳杆菌SB27降低NDMA对大鼠肠道黏膜细胞IEC-6毒性作用能力进行分析。结果 CCK-8实验结果显示, 干酪乳杆菌SB27可减小NDMA致大鼠肠道黏膜细胞IEC-6毒性的影响, 且该作用与干酪乳杆菌SB27的剂量有关。HE染色、TUNEL染色和透射电镜实验结果显示, NDMA (80 μg/mL)诱导了IEC-6细胞的损伤, 干酪乳杆菌SB27 (104~106 CFU/mL)可保护IEC-6细胞免受NDMA的毒性损伤。结论 干酪乳杆菌SB27可降低NDMA对大鼠肠道黏膜细胞IEC-6的毒性损伤。

英文摘要:

      Objective To investigate the ability of Lactobacillus casei (SB27) to reduce the toxicity of N-nltrosodimethylamine (NDMA) on intestinal mucosal cells IEC-6 in rats. Methods The ability was analyzed by CCK-8 assay, hematoxylin-eosin (HE) staining, TUNEL staining and transmission electron microscopy, which could reduce the toxicity of Lactobacillus casei SB27 on intestinal mucosal cells IEC-6 in rats induced by NDMA. Results The results of CCK-8 assay showed that Lactobacillus casei SB27 could reduce the toxicity of intestinal mucosal cells IEC-6 induced by NDMA, and the effect was related to the dose of Lactobacillus casei SB27. The results of HE staining, TUNEL staining and transmission electron microscopy showed that NDMA (80 μg/mL) induced the damage of IEC-6 cells, and Lactobacillus casei SB27 (104-106 CFU/mL) could protect IEC-6 cells from the toxic damage of NDMA. Conclusion Lactobacillus casei SB27 can reduce the toxicity damage of NDMA to rat’s intestinal mucosal cells IEC-6.