近红外旋光法检测食糖的蔗糖分

Determination of sucrose in sugar by near-infrared polarimetry

中文关键词:  近红外旋光法  食糖  蔗糖分  二次旋光法  旋光度

英文关键词:near-infrared polarimetry  sugar  sucrose  two rotatory analysis  optical rotation

中文摘要:

      目的 建立近红外旋光法测定食糖中蔗糖分的分析方法。方法 通过配制纯蔗糖溶液, 确定在589.44 nm (589 nm)和880.00 nm (880 nm)波长下蔗糖溶液酸水解前后旋光度差值的比值, 基于普通旋光法(国家标准)推导出近红外旋光法计算蔗糖分公式, 并将测定结果进行比对。结果 在589 nm和880 nm波长下蔗糖溶液酸水解前后旋光度差值的比值为2.333, 在蔗糖浓度为50%~100%时, 近红外旋光法线性关系良好, 回收率为98.9%~100.1%, 相对标准偏差为0.040%~0.064%, 与普通旋光法比对结果一致。结论 该方法灵敏、准确, 具有良好的重复性和稳定性, 应用范围广, 可用于食糖的蔗糖分检测。

英文摘要:

      Objective To establish a method for the determination of sucrose in sugar by near-infrared polarimetry. Methods By preparing pure sucrose solution, the ratio of optical rotation difference before and after acid hydrolysis of sucrose solution was detemined at wavelengths of 589.44 nm (589 nm) and 880.00 nm (880 nm). Based on ordinary polarimetry (national standard), the formula for calculating sucrose by near-infrared polarimetry was deduced, and the measurement results were compared. Results The ratio of optical rotation difference before and after acid hydrolysis of sucrose solution at wavelengths of 589 nm and 880 nm was 2.333. The linear relationships of near-infrared polarimetry within 50%-100% sucrose concentrationwere good, the recoveries were between 98.9% and 100.1%, the relative standard deviations were between 0.040% and 0.064%, which were consistent with the results of ordinary polarimetry. Conclusion The method is sensitive, accurate, has good repeatability and stability, has a wide range of applications, and can be used for the detection of sucrose in sugar.