西洋参水提物改善睡眠功能的研究

Study on improving sleep function of water extract of Panax quinquefolium

中文关键词:  西洋参水提物  睡眠  戊巴比妥钠  巴比妥钠

英文关键词:water extract of Panax quinquefolium  sleep  pentobarbital sodium  barbital sodium

中文摘要:

      目的 研究不同剂量西洋参水提物对小鼠睡眠功能的影响。方法 根据改善睡眠功能评价方法, 分别通过直接睡眠实验、延长戊巴比妥钠睡眠时间实验、戊巴比妥钠阈下剂量催眠实验、巴比妥钠睡眠潜伏期实验判定西洋参水提物高、中、低剂量组[133.3、66.7、33.3 mg/(kg·BW)相当于人体剂量的20、10、5倍]是否具有改善小鼠睡眠的功能, 并通过酶联免疫吸附分析(enzyme linked immunosorbent assay, ELISA)实验测定小鼠脑干中5-羟色胺(5-hydroxytryptamine, 5-HT)的含量。结果 西洋参水提物各剂量组均无直接睡眠作用; 与空白对照相比, 西洋参水提物中、高剂量组能显著延长小鼠睡眠时间(P<0.05), 提高小鼠的入睡率(P<0.05), 且西洋参水提物高剂量组能显著缩短小鼠巴比妥钠睡眠潜伏期, 并能显著提高小鼠脑干中5-HT含量(P<0.05)。结论 西洋参水提物具有改善小鼠睡眠的作用, 这可能与其提高小鼠脑干中5-HT含量有关。

英文摘要:

      Objective To study the effects of different doses of water extract of Panax quinquefolium on sleep in mice. Methods According to the improved sleep function evaluation method, direct sleep test, prolonged pentobarbital sodium sleep time test, pentobarbital sodium subthreshold dose hypnosis test and barbituric sodium sleep latency test were carried out respectively to determine whether the water extract of Panax quinquefolium in high, medium and low dose groups [133.3, 66.7, 33.3 mg/(kg·BW) were equivalent to 20, 10 and 5 times of human dose] had the function of improving sleep in mice. The content of 5-hydroxytryptamine (5-HT) in brain stem of mice was determined by enzyme linked immunosorbent assay (ELISA) experiment. Results The water extract of Panax quinquefolium had no direct sleep effect in all dosage groups, compared with blank control, medium and high dose of Panax quinquefolium water extract groups could significantly prolong the sleep time of mice (P<0.05) and improve the sleep rate of mice (P<0.05), high dose of Panax quinquefolium water extract group could significantly shorten the sleep latency of barbital sodium in mice, and significantly increase the content of 5-HT in brain stem of mice (P<0.05). Conclusion The water extract of Panax quinquefolium can improve the sleep of mice, which may be related to increasing the content of 5-HT in brain stem of mice.